摘要
目的：建立高效液相色谱法测定茯苓配方颗粒中茯苓酸的含量。方法：采用HPLC法,乙腈-0.5%磷酸溶液（83∶17）为流动相,流速为1.0 ml/min,检测波长为210 nm,柱温为35℃。结果：6个厂家12批次的茯苓配方颗粒中茯苓酸含量介于0.87-69.13μg/g之间。结论：该方法操作简便,结果稳定可靠,可用于茯苓配方颗粒的质量评价。
Objective： To establish an HPLC method for determination of pachymic acid in poria formula granule. Methods：HPLC was performed on a Diamonsil C18（ 4. 6 mm × 250 mm,5 μm） column at 35 ℃ with mobile phase consisting of acetonitrile-0. 5% phosphoric acid solution（ 83∶ 17）. The flow rate was 1. 0 ml / min. The detection wavelength was 210 nm. Results： The content of pachymic acid in twelve batch es of poria formula granule products from six factories ranged between 0. 87 μg / g and69. 13 μg / g. Conclusion： The method is simple and reliable,and can be used for the quality evaluation of poria formula granule.
出处
《天津药学》
2015年第6期16-17,共2页
Tianjin Pharmacy
基金
天津市科技支撑计划重点项目(No.12ZCZDSY01000)
