葡萄霜霉病菌拮抗放线菌PY-1发酵条件优化

Optimization of Fermentation Conditions for Antagonistic Actinomycete PY-1 against Plasmopara viticola

为探讨暗黑链霉菌Streptomyces atratus PY-1液体摇瓶发酵条件,提高其活性次级代谢产物的产量,以菌体生物量和发酵液抑制葡萄霜霉病菌Plasmopara viticola活性为指标,采用单因子试验和正交试验对菌株PY-1的最适发酵培养基成分及发酵条件进行优化。结果表明,菌株PY-1最适发酵培养基为玉米粉50 g/L、葡萄糖5 g/L、蛋白胨5 g/L、氯化铵5 g/L、氯化钠0.5 g/L;最佳发酵培养条件为培养温度28℃、培养时间5 d、初始p H 7.0、250 m L三角瓶装液量90 m L、接种量体积分数5%、摇床转速180 r/min。在最佳发酵培养基和培养条件下,菌株PY-1发酵液抑菌率达到99.26%,抑菌能力提高8.35%。

Authors used single factor experiments and orthogonal experiments to optimize the liquid fermentation conditions of Streptomyces atratus PY-1 to increase its antagonistic substance productivity against Plasmopara viticola. The result showed that the optimum medium was corn meal 50 g, glucose 5 g, peptone 5 g, NH4 Cl 5 g, Na Cl 0.5 g, in 1 liter of distilled water. The optimal incubation conditions were 28 ℃, fermentation time 120 h, initial p H 7.0, medium capacity 90 m L/250 m L, inoculation rate 5%, and rotate speed 180 r/min. The inhibitory activity of strain PY-1 fermentation solution was increased by 8.35% under the optimum fermentation medium and incubation conditions.