粗壮脉纹孢菌降解豆皮粗纤维产可发酵糖培养基优化及单糖分析

Optimization of Medium Composition for Soybean Hull Fiber Degradation by Neurospora crassa and Monosaccharide Composition Analysis of the Resulting Fermentable Sugars

研究考察了粗壮脉纹孢菌固态发酵豆皮酒糟培养基产纤维素酶的最优培养基组成,并用气相色谱法对该发酵条件下豆皮纤维素降解所产的可发酵糖进行定性检测。结果表明:产纤维素酶的最优培养基组成为豆皮64%、酒糟34%、(NH4)2SO4 2%,在此培养基中粗壮脉纹孢菌发酵产纤维素酶的酶活力为2.83 IU/g,较优化前的1.17 IU/g提高了141.88%,还原糖产量为18.01 g/100 g,较优化前9.91 g/100 g提高了81.74%。获得的可发酵糖的单糖组成主要为葡萄糖和木糖。

In this work, Plackett-Burman design and response surface methodology were applied to optimize the solid medium containing soybean hull and distillers＇ grains to produce cellulase by Neurospora crassa. Meanwhile, the monosaccharide composition of fermentable sugars from the degraded product under optimal culture medium was analyzed by gas chromatography. The results showed that the optimal medium was composed of 64% soybean hull, 34% distillers＇ grains and 2% ammonia sulfate. Under the optimal condition, the activity of cellulase significantly was improved by 141.88%, from 1.17 IU/g to 2.83 IU/g, and the yield of reducing sugar was increased by 81.74%, from 9.91 g/100 g to 18.01 g/100 g compared to that obtained with the unoptimized medium. The major monosaccharide components of the resulting fermentable sugars were glucose and xylose, respectively.