海湾扇贝多肽混合物对人肝癌HepG2细胞增殖和凋亡的影响

Effect of Polypeptide Mixture from Bay Scallop on Proliferation and Apoptosis of HepG2 Cells

采用CCK-8(Cell Counting Kit-8)法检测海湾扇贝多肽混合物对体外培养的人肝癌HepG2细胞增殖的抑制作用,利用倒置显微镜及流式细胞仪观察、分析其对HepG2细胞的形态和细胞凋亡的影响。结果表明:不同质量浓度海湾扇贝多肽混合物(4、6、8、10、12 mg/m L)能够抑制人肝癌HepG2细胞的生长,且抑制率随海湾扇贝多肽混合物质量浓度的增加而升高。经12 mg/m L海湾扇贝多肽混合物处理后,HepG2细胞的形态发生了明显改变,表现为细胞皱缩、透明度降低、细胞核固缩等,并出现了凋亡小体。海湾扇贝多肽混合物可诱导Hep G2细胞凋亡。

The antitumor efficacy and mechanism of the polypeptide mixture from bay scallop were studied by CCK-8 assays using inverted microscope and fluorescence-activated cell sorter（FACS）. The results showed that different concentrations of the polypeptide mixture from bay scallop（4, 6, 8, 10 and 12 mg/m L） could suppress the growth of human hepatocellular carcinoma cells HepG2, and the growth inhibitory rate increased with increasing concentration of the polypeptide mixture. After treated with the polypeptide mixture at a dose of 12 mg/m L, the morphology of HepG2 cells was changed, showing cell shrinkage, decreased transparency and nuclear chromatin pyknosis as well as the appearance of apoptotic bodies. To conclude, the polypeptide mixture from bay scallop can induce apoptosis of HepG2 cells.