鼻息肉术后增生组织中转化生长因子α、β_1的表达和激素的作用

The expression of transforming growth factor α,β_1 in hyperplastic tissue after endoscopic polypectomy and the effect of corticosteroid

目的 :研究鼻息肉术后增生组织中转化生长因子α(transforminggrowthfactorα ,TGFα)、β1 转化生长因子 β1 (TGFβ1 )的表达及皮质类固醇对其表达的影响。方法 :将 4 0例鼻息肉病患者随机分为两组 :激素组 :术后 1周开始应用布地奈德 (BUD)鼻腔喷雾 (40 0 μg d ) ;对照组 :术后不使用BUD及其它类固醇药物。经鼻内窥镜鼻息肉切除术后第 1、8周门诊复查时钳取筛窦腔内的增生组织。采用HE染色观察增生组织的形态学改变 ;以免疫组织化学方法观察两组增生组织中TGFα、TGFβ1 的表达。结果 :术后增生组织的形态学改变与鼻息肉相类似。对照组增生组织上皮细胞、炎性细胞、部分腺上皮细胞TGFα高表达 ,炎性细胞TGFβ1 高表达。BUD组增生组织中TGFα、TGFβ1 的表达明显下降 (P <0 .0 1,0 .0 5 )。结论 :TGFα和TGFβ1

Objective:To study the expression of transforming growth factor α, β 1 in hyperplastic tissue after endoscopic polypectomy and the effect of corticosteroid. Method:Forty patients with nasal polyps were divided into two groups randomly: corticosteroid group (n=20) with topical application of Budesonide (BUD, 400 μg/d) after endoscopic polypectomy and control group (n= 20) without corticosteroid after surgery. The hyperplastic tissues in operative cavity obtained in the 1st and 8th weeks after operation were studied with HE staining and immunohistochemistry technique respectively. Result:Morphological changes of hyperplastic tissue after endoscopic polypectomy included pseudostratified epithelium, highly edematous lamina proper and inflammatory cells infiltration, in which the main infiltrative cells were eosinophils ( 67.5 %). Transforming growth factorα(TGFα) protein was highly expressed in epithelial cells, grand cells and inflammatory cells in the hyperplastic tissue. Transforming growth factorβ 1 (TGFβ 1) protein was highly expressed in inflammatory cells in the hyperplastic tissue. The expression of TGFα and β 1 was significantly decreased after topical BUD spray (P< 0.01 , 0.05 ).Conclusion:Transforming growth factor α and β 1 may play an important role in the formation and recurrence of nasal polyps.