稀土纳米金对银染效果的影响及其UV-Vis吸收光谱（英文）

Effect of REAu on Silver Staining Results and Their UV-Vis Absorption Spectra

制备了La,Ce,Nd,Sm,Eu,Gd,Dy微粒和纳米金,分别用La,Ce,Nd,Sm,Eu,Gd,Dy微粒替代部分的纳米金,研究了La-Au,Ce-Au,Nd-Au,Sm-Au,Eu-Au,Gd-Au,Dy-Au微粒分别对银染效果的影响及其紫外可见（UV-Vis）吸收光谱。与纳米金相比,La-Au,Ce-Au,Nd-Au,Sm-Au,Eu-Au,Gd-Au,Dy-Au微粒可延长银染后的斑点持续的时间,其中Nd-Au微粒的效果最好,斑点持续的时间为30min,是纳米金的2.7倍;可大幅度加深斑点的颜色,其中Nd-Au,Sm-Au微粒的效果最好,用Nd微粒替代部分的纳米金,纳米金用量降低了80%,但还能提高银染法的灵敏度。在200~800nm范围,La,Ce,Nd,Sm,Eu,Gd,Dy微粒和纳米金溶液的UV-Vis吸收光谱只有一个吸收峰,λmax分别为275,277,276,276,278,277,278和521nm;La-Au,Ce-Au,Nd-Au,Sm-Au,Eu-Au,Gd-Au,Dy-Au微粒混合液的UV-Vis吸收光谱有两个吸收峰,λmax（RE）和λmax（Au）分别为276和522nm,276和522nm,276和523nm,276和523nm,276和522nm,276和522nm,276和523nm,纳米金和La微粒的吸收峰的波长发生了红移,Ce,Eu,Gd,Dy微粒的吸收峰的波长发生了蓝移,Nd,Sm微粒的吸收峰的波长不变,纳米金与稀土微粒可能有相互作用。

La, Ce, Nd, Sm, Eu, Gd, Dy particles and gold nanoparticles were prepared. Effects of La-Au, Ce-Au, Nd-Au, Sm-Au, Eu-Au, Gd-Au and Dy-Au particles on silver staining results were studied, respectively, and UV-Vis absorption spectra of La-Au, Ce-Au, Nd-Au, Sm-Au, Eu-Au, Gd-Au and Dy-Au parti- cles were studied. Times and colors of sports with La-Au, Ce-Au, Nd-Au, Sm-Au, Eu-Au, Gd-Au, Dy-Au particles are longer and darker than that of with gold nanoparticles, respectively. The time of sport with NdAu particles is as long as 30 min, which is 2.7 times as long as with gold nanoparticles. Although amount of gold nanoparticles reduced 80%, the color of sport with Nd-Au particles is darker than that of with gold nanoparticles. In 200.00-800.00 nm, La, Ce, Nd, Sin, Eu, Gd, Dy particles and gold nanoparticles has one ab- sorption peak, respectively, and λmax is 275, 277, 276, 276, 278, 277, 278 and 521 nm, respectively. La-Au, Ce-Au, Nd-Au, Sm-Au, Eu-Au, Gd-Au, Dy-Au particles have two absorption peaks, respectively, λmax（RE） and λmax（Au） are 276 and 522 nm, 276 and 522 nm, 276 and 523 nm, 276 and 523 ran, 276 and 522 rim, 276 and 522 nm, 276 and 523 nm, respectively. λmax of Au nanoparticles and La particles occurs red moving respectively, and λmax of Ce, Eu, Gd and Dy particles occurs blue moving, respectively, and 3. of Nd and Sm particles is constant respectively. Rare earths particles and gold nanoparticles may have interaction, respectively.