高危型人乳头瘤病毒整合态与宫颈病变相关性研究

Correlation between high-risk human papillomavirus integration and cervical lesions

目的通过随访1年观察整合态高危型人乳头瘤病毒(HR-HPV)进展为宫颈高级别病变的患病率探讨宫颈上皮中整合态HR-HPV与宫颈病变发生发展的关系。方法利用实时荧光聚合酶链反应(PCR)方法检测96例HR-HPV组和宫颈上皮内瘤变(CINI)组中宫颈脱落细胞中HR-HPV DNA的整合状态。结果①2组进展为高级别的宫颈病变的患病率比较差异有统计学意义。②在宫颈病变有关因素的分析中,整合态HR-HPV感染时发生宫颈病变的危险度增加。结论整合态HR-HPV感染为引起宫颈高度恶性病变的主要致病因素。

Objective To determine the prevalence of progression from the high-risk human papillomavirus（HR-HPV） integration to high-grade cervical lesions during a one-year follow-up,and to investigate the correlation between integrated HR-HPV in cervical epithelium and the occurrence and development of cervical lesions.Methods Real-time fluorescent PCR was used to determine the integration of HR-HPV DNA in cervical exfoliated cells in HRHPV group and cervical intraepithelial neoplasia（CIN I） group（n =96 each）.Results① There was a statistically significant difference in the prevalence of progression from HR-HPV integration to high-grade cervical lesions.②Analysis of cervical lesions related factors showed that the risk of cervical lesions increased with infection of integrated HR-HPV.Conclusion Infection of integrated HR-HPV is the main factor causing high-grade cervical lesions.