吉西他滨联合乙酰肝素酶抑制剂对胰腺癌PANC-1细胞侵袭和迁移的影响

Effects of gemcitabine combined with heparanase inhibitor on invasion and migration of pancreatic cancer PANC-1 cells

目的探讨吉西他滨(GEM)联合乙酰肝素酶抑制剂OGT2115对胰腺癌PANC-1细胞侵袭和迁移能力的影响。方法人胰腺癌PANC-1细胞于RPMI 1640培养基中培养24 h后采用随机数字表法分为4组;对照组(C组)、GEM组、OGT2115组、GEM+OGT2115组分别通过Transwell小室细胞侵袭活性实验和细胞划痕实验检测各组PANC-1细胞侵袭和迁移的能力,通过酶联免疫吸附试验(ELISA)法检测各组乙酰肝素酶(HPA)的活性。结果与C组比较,GEM组、OGT2115组、GEM+OGT2115组侵袭细胞数和细胞迁移率降低(P<0.05);与GEM组和OGT2115组比较,GEM+OGT2115组侵袭细胞数和细胞迁移率降低(P<0.05);与C组和GEM组比较DGT21 15组HPA活性降低(P<0.05),OGT21 15+GEM组HPA活性降低(P<0.05)。结论吉西他滨联合乙酰肝素酶抑制剂可抑制胰腺癌PANC-1细胞的侵袭和迁移,OGT2115可能通过抑制HPA的活性而增强吉西他滨抗胰腺癌PANC-1细胞侵袭和迁移的能力。

Objective To investigate the effects of gemcitabine（GEM） combined with the heparanase inhibitor OGT2115 on invasion and migration of pancreatic cancer PANC-1 cells.Methods Human pancreatic cancer PANC-1 cells were cultured in RPMI 1640 medium,and randomly divided into four groups after 24 h：control group（group C）,GEM group,OGT2115 group,and GEM＋OGT2115 group,respectively.The capacities of PANC-1 cell invasion and migration in each group were measured with Transwell chamber experiments and cell scratch assay.The activity of heparanase（HPA） was detected by ELISA.Results Compared with group C,the number of invasive cells and cell migration rate in the GEM group,OGT2115 group,GEM ＋ OGT2115 group were decreased（P〈0.05）.Compared with GEM group and OGT2115 group,the GEM ＋ OGT2115 group showed fewer invasive cells and lower cell migration rate（P〈0.05）.Compared with group C and GEM group,the HPA activity was decreased in OGT2115 group and GEM＋OGT2115 group（P〈0.05）.Conclusion Gemcitabine combined with heparanase inhibitor may inhibit the invasion and migration of pancreatic cancer PANC-1 cells.OGT2115 may inhibit the activity of HPA and thereby enhance the actions of gemcitabine against invasion and migration of pancreatic cancer PANC-1 cells.