基于多级错流提取槟榔生物碱的工艺优化

Study on optimal conditions for extracting alkaloids from areca catechu based on multistage cross flow extraction

为了优化槟榔生物碱的提取工艺,以青果槟榔壳为原料,采用多种溶剂体系进行提取,确定最佳提取溶剂;根据多级错流浸取理论公式设计料液比和浸取级数,在单因素试验的基础上,以提取温度、提取时间、溶剂pH值为自变量,以槟榔生物碱提取率为指标,进行有交互的正交试验。结果表明:以水为提取剂,料液比1:20(m:V),提取温度60℃,提取时间100min,pH值为2,提取2次,该条件下的槟榔生物碱提取率为7.241 7mg/g。

In order to optimize the extraction process of alkaloids from areca catechu,the alkaloids was extracted by varied extraction solvent systems from areca catechu shell and the optimal extraction solvent was explored.According to the theoretical formula of multistage cross flow extraction,solid to liquid ratio and extractive grades was designed.Based on the results of single-factor test,the effect of extraction temperature,extraction time,pH value on extraction rate were conducted by orthogonal test of interaction.The result showed that the extraction rate of alkaloids was 7.241 7mg/g under the optimal conditions：taking water as the solvent,solid to liquid ratio 1︰20（m ︰V）,extraction temperature 60℃,extraction time 100min;pH value,2,extraction times 2.