N＋注入番茄红素生产菌株的选育及基因转录分析

Breeding of lycopene high producing strain by N^+ implantation and analysis of gene transcription

对三孢布拉霉(Blakeslea trispora)负菌进行了N+诱变,筛选出1株高产番茄红素的负菌株4c,并研究了出发菌株与高产菌株番茄红素合成关键基因表达量的变化。研究表明,突变株番茄红素合成关键基因hmgR、car B和carRA的表达量与出发菌株相比分别提高了2.2倍、2.35倍和2.16倍;与正菌混合发酵48 h后hmgR、car B和carRA的表达量与出发菌株相比分别提高了2.0倍、1.52倍和1.35倍。突变株与正菌共培养发酵96 h后,番茄红素产量达到了25.97 mg/g,与出发菌株相比,提高了39%。

Blakeslea trispora （ - ） strain, the main lycopene producer, was treated with N~ implatation. After several rounds of selection, a strain named 4c with high lycopene production was obtained. The differences of the transcriptional level of the key genes involved in lycopene biosynthesis were measured between parent and mutant strains. Compared with parent strain, the transcription level of the key genes hmgR, carB and carRA in mutant strain increased by 2.2, 2.35 and 2.16 times, respectively. The transcription level of the key genes in mutant strain after 48 h mixed culture with the （ ＋ mentation with the （ ＋ ） strain, the parent strain （18.66 rag/g） ） strain increased by 2.0, 1.52 and 1. a maximum lycopene yield reached 25. 35 times, respectively. After 96 h mixed fer- 97 mg/g, which was 39% higher than that of