响应面优化大孔吸附树脂分离纯化茼蒿总黄酮

Purification of total flavonoids from Chrysanthemum coronarium L.with macroporous resins by response surface analysis

为了优化大孔吸附树脂分离纯化茼蒿总黄酮(TFC)的工艺条件,试验以TFC吸附量和回收率为指标,考察5种大孔吸附树脂静态、动态的吸附及解吸附性能。优选影响因素——上样浓度,上样p H值,洗脱浓度和洗脱流速4种试验条件,测定大孔树脂的泄露曲线和洗脱曲线。研究发现,D-101大孔树脂对TFC的吸附纯化显示出最佳的性能。泄露曲线显示:上样体积泄漏点60 m L,饱和点210 m L。洗脱曲线显示:体积分数95%乙醇溶液80 m L洗脱效果最好。响应面试验方差分析显示:上样p H对TFC回收率影响差异达到显著水平,而上样浓度、洗脱浓度和洗脱速度3个因素对TFC回收率影响差异不显著。通过回归方程模拟预测最优纯化条件组合——上样浓度0.86mg/m L,p H=6,洗脱浓度74.27%,洗脱流速2.23 m L/min,TFC回收率由65.19%提升至87.36%。D-101大孔树脂对于TFC的分离纯化性能较好,适用于工业化推广。

To optimize the technical condition for separation and purification of total flavonoids of Chrysanthemum coronarium L. （TFC） with macroporous resin is the experiment goal. With adsorption capacity and recovery rate of TFC as index, the adsorption and desorption of five kinds maeroporous resin at static and dynamic conditions were an- alyzed. Concentration and pH of sample, concentration and flow rate of elution were studied as the four major factors in the experiment. The experiment also measured leakage curve and elution curve of macroporous resins. Response surface was designed with four factors and three levels according to central composite experimental principle. Results show that the D-101 maeroporous resin is the best macroporous resin to separate and purify the TFC. The leakage curve show that sample volume leakage point is 60 mL and saturation point is 210 mL. The elution curve show that the 95% ethanol with 80 mL is the best condition. Response surface analysis of variance show that the effect of sample＇ s pH on the recovery rate of TFC is significant, but the effect of concentration of sample and elution and elution rate on the recovery rate of TFC is not significantly. Through regression equation to predict the optimal combination of pur- ification is sample concentration 0.86mg / mL, pH = 6, elution concentration 74.27% ,elution flow rate 2.23 mL/ rain, TTFC＇ s recovery increased from 65. 19% to 87.36%. D-101 maeroporous resin have good performance for sep- aration and purification of TFC. It is worthy industrial promotion.