一株醚苯磺隆降解菌的分离、鉴定及固定化应用研究

ISOLATION,IDENTIFICATION AND IMMOBILIZED APPLICATION OF A TRIASULFURON DEGRADING STRAIN

从某农药厂的活性污泥中富集、筛选出一株醚苯磺隆的共代谢降解菌株MB-1,鉴定为铜绿假单胞菌(Pseudomonas aeruginosa)。研究碳源、醚苯磺隆初始浓度及接菌量对菌株降解特性的影响,实验结果表明:葡萄糖为200 mg/L,醚苯磺隆初始浓度为200 mg/L,接菌量为1%时,MB-1对醚苯磺隆的降解效果最佳。采用海藻酸钠固定化技术对菌株MB-1进行固定化。结果表明海藻酸钠质量分数为4%,CaCl_2质量分数为2%,接菌量为2%,钙化时间为4 h时,固定化菌株对醚苯磺隆废水的降解效果良好。将固定化MB-1小球投入实验室规模的SBR装置中进行应用发现,固定化MB-1菌株可以持续高效降解醚苯磺隆废水。

A triasulfuron-degrading strain MB-1 was isolated from the contaminated active sludge collected from wastewatertreating system in a pesticide manufacturer,which was identified as Pseudomonas aeruginosa. The effects of different carbon sources,initial triasulfuron concentration and inoculation amount on degradation efficiency were determined. The results revealed the glucose of 200 mg / L,initial triasulfuron concentration of 200 mg / L and inoculation amount of 1% were best for its biodegradation efficiency. Using sodium alginate immobilization technology for immobilizing MB-1,experimental results showed sodium alginate content of 4%,CaCl_2 content of 2%,inoculation amount of 2% and calcification time of 4 h were best for immobilizing strain MB-1 for triasulfuron biodegradation. In laboratory-scale sequencing batch reactor（ SBR） experiment,the immobilized bacteria MB-1 showed stable and efficient performance for degrading trisulfuron for a long period.