花生青枯病抗性相关SSR标记的筛选鉴定

Identification of SSR makers associated with bacterial wilt resistance of peanut through correlation analysis

利用72对多态性SSR引物对57份具有不同青枯病抗性的花生品种进行遗传多样性分析,结合青枯病抗性鉴定结果,利用Pearson＇s相关和多元线性逐步回归鉴定与花生青枯病抗性相关的SSR标记。SSR标记多态性分析表明,57份花生品种遗传相似系数为0.497 8-0.960 7,平均0.703 8。在遗传相似系数为0.69处,参试品种聚类为2个类群,与品种的青枯病抗性鉴定结果和品种的植物学类型基本一致,抗病品种之间的遗传差异大于感病品种之间的差异。相关分析和多元线性逐步回归分析表明,共有10个标记位点与青枯病抗性显著相关,其中1B9-3、IPAHM288-1和ARS590-1与青枯病抗性极显著关联。用这3个标记检测RIL群体,标记位点1B9-3和ARS590-1的符合率分别为62.85%和40.00%,标记IPAHM288在群体中无差异。

Bacterial wilt caused by Ralstonia solanacearum is one of the important diseases limiting the production of peanut（ Arachis hypogaea L.） in China. In the present study,72 polymorphic SSR markers were used to detect the genetic diversity of 57 peanut cultivars with various resistance to bacterial wilt（ BW）. The results showed that the coefficients of genetic similarity between two genotypes among the involved 57 cultivars varied from0. 4978 to 0. 9607 with the average of 0. 7038. According to UPGMA cluster analysis,the involved 57 cultivars were clustered into two groups when the threshold valve of coefficients of genetic similarity was 0. 69. Group I contained 30 accessions including 23 hypogaea genotypes and 7 vulgaris genotypes. 29 of the 30 accessions in group I were susceptible to BW. Group II composed of 27 vulgaris genotypes,among which,26 were resistant to BW and one was susceptible. The clustering result of the involved 57 cultivars by SSR data was consistent with the botanical type and BW resistance of the cultivars. The genetic diversity among cultivars with BW resistance was slightly greater than that of the susceptible cultivars involved in the present study. Combining the data from bacterial wilt evaluation of the genotypes in two years,a total of 10 SSR loci related to BW resistance were detected through correlation analysis and multiple linear regression analysis,among which,3 loci including 1B9- 3,IPAHM288- 1 and ARS590- 1 were highly significantly associated with BW resistance. These 3 highly significantly associated with BW resistance makers were used to detect the RIL population from crossing of parents with BW resistance and susceptible respectively,loci 1B9- 3 and ARS590- 1 were detected in 62. 85% and 40. 00% of the BW resistant lines.