大岩桐花萼和幼叶转录组研究

Sequencing and Characterization of Transcriptome of Sinningia speciosa Sepals and Young Leaves

利用新一代高通量测序技术平台Illumina Solexa GAⅡx对大岩桐花萼和幼叶进行转录组测序和数据从头组装及生物信息学分析,获得了长度大于100 bp的转录物22 821条;转录本总长度28.63Mb,N50长度1 548 bp,序列平均长度953 bp。转录物注释结果显示,15 053条有同源比对信息;7 768条无匹配序列信息,可能为大岩桐特有的基因序列。通过对花萼、幼叶中与激素代谢、信号转导相关的差异KEGG通路比较,推断在花萼中促进生长激素合成基因的表达比幼叶中强,激素信号转导组分基因的表达也比幼叶中强。

The transcriptome of Sinningia speciosa sepals and young leaves was sequenced by using Illumina Solexa GAⅡx,and the functional annotations and metabolic pathway analyses of some unigenes were conducted in this study. And 22 821 unigenes,longer than 100 bp,were obtained by de novo assembly method. The total scaffold sequence length reached 28.63 Mb with the 1 548 bp of N50,the average unigenes length was 953 bp. Annotation analysis of unigene indicated that 15 053 unigenes had homolog in public protein database;However,7 768 sequences were no hit and might be Sinningia speciosa specific. The study of different KEGG pathways related to hormone metabolism and signaling in sepals and young leaves,inferred that the expression of hormone synthesis genes of promoting growth in sepals is stronger than in leaves,so was the expression of the signal transduction component genes.