miR-328、miR-147和miR-22在冠心病患者中的表达变化及临床意义

Differential Expression and Clinical Significance of miR-328,miR-22 and miR-147 in Coronary Heart Disease Patients

目的探讨冠心病患者血浆及外周血单个核细胞(PBMC)miR-328、miR-22、miR-147的表达变化及其临床意义。方法筛选100例冠心病患者及相对健康对照组,采用实时荧光定量PCR技术检测各组血浆及PBMC中miR-328、miR-147、miR-22的表达水平。结果冠心病患者血浆中miR-328、miR-22的表达较非冠心病患者明显升高,而miR-147的表达明显降低;在冠心病患者PBMC中,miR-328表达没有明显变化,miR-22表达略微增加,miR-147表达明显下降。在心肌梗死患者中,血浆中miR-328和miR-22表达水平明显增加,而miR-147表达明显下降;心肌梗死患者PBMC中miR-328表达略降低,miR-22表达增加,miR-147表达水平略有下降。结论 miR-328、miR-22及miR-147在冠心病患者中的差异表达,有望作为冠心病疾病中一种新的标记物或基因治疗靶点。

Aim To explore the expression level and clinical significance of miR-328,miR-22 and miR-147 in coronay heart disease（ CHD） patients in plasma and peripheral blood mononuclear cells（ PBMC）. Methods 100 cases with CHD and healthy volunteers were screened; Expression of miR-328,miR-22 and miR-147 in plasma and PBMC in different groups was detected by real-time quantitative PCR. Results Compared with the patients with non-CHD,the plasma expression of miR-328 and miR-22 in patients with CHD significantly increased,and the plasma expression of miR-147 in patients with CHD significantly decreased; Compared with the non-CHD,the expression of miR-328 in PBMC had no significant difference in CHD group,and the expression of miR-22 in PBMC was up-regulated in CHD group,but the expression of miR-147 in PBMC was obviously down-regulated; Compared with the patients with non-AMI,the expression of miR-328 and miR-22 in plasma was significantly increased in AMI,but the expression of miR-147 in plasma was obviously decreased in AMI; In addition,the miR-328 and miR-147 levels in PBMC in AMI patients were slightly lower,and the miR-22 level was elevated in PBMC in AMI patients. Conclusion Significantly differently expressed miR-328,miR-22 and miR-147 are expected to be new cardiovascular disease markers or gene therapeutic targets in CHD patients.