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蒙古柳cDNA转化酵母菌及NaCl胁迫抗性基因筛选

Full-length cDNA of Salix linearistipularis Transformed into Yeast and Resistant Genes Identified under NaCl Stress
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摘要 为挖掘蒙古柳的NaCl胁迫相关基因,本研究利用FOX hunting system技术,构建蒙古柳cDNA连接pYES2载体后转入酵母InVSCI菌株。本研究将pYES2-蒙古柳cDNA酵母菌在1.0 mol/L Na 抗性板胁迫处理后得到15个抗NaCl胁迫的酵母菌株,并利用这15个菌株进一步通过NaHCO3、H2O2和Srobitol抗性进行分析,结果发现都表现出比对照组更强的耐胁迫性。接着对这15个菌株提取细胞DNA后克隆获得蒙古柳cDNA,在NCBI数据库中对测序得到的基因序列进行相似性比对,结果发现大部分和毛果杨基因有高的相似性,其中一些基因已有研究表明和盐胁迫相关。本研究可为挖掘可利用的耐盐基因资源提供依据。 In order to select Na Cl stress related genes of Salix linearistipularis, using FOX hunting system technology, double-stranded c DNA ligated into p YES2 vector and transformed into a yeast strains（In VSCI）. The138 resistance yeast was isolated from p YES2-c DNA yeast in 1.0 mol/L Na Cl stress treatment, and further resistance after 1.0 mol/L NaCl, 15 yeast strains was obtained. The resistance of 15 strains was more higher than the control by NaHCO3, H2O2 and Srobitol stresses. After sequencing, these gene sequences had higher similarity with Populus trichocarpa, some of the genes were shown to salt stress related genes. This research can provide the basis for mining use of salt tolerant gene resources.
作者 南桂仙 乔坤 高野哲夫 柳参奎
机构地区 东北油田盐碱植被与恢复教育部重点实验室 延边大学农学院 东京大学亚洲生物资源环境研究中心
出处 《分子植物育种》 CAS CSCD 北大核心 2015年第5期1123-1129,共7页 Molecular Plant Breeding
基金 中国教育部科研创新团队计划(IRT13053)资助
关键词 蒙古柳(Salix linearistipularis) 全长CDNA 酵母转化 抗性基因 NACL胁迫 Salix linearistipularis Full length cDNA Yeast transformation Resistant gene NaCl stress
分类号 S792.12 [农业科学—林木遗传育种]
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参考文献13

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分子植物育种
2015年 第5期

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