摘要
为挖掘蒙古柳的NaCl胁迫相关基因,本研究利用FOX hunting system技术,构建蒙古柳cDNA连接pYES2载体后转入酵母InVSCI菌株。本研究将pYES2-蒙古柳cDNA酵母菌在1.0 mol/L Na 抗性板胁迫处理后得到15个抗NaCl胁迫的酵母菌株,并利用这15个菌株进一步通过NaHCO3、H2O2和Srobitol抗性进行分析,结果发现都表现出比对照组更强的耐胁迫性。接着对这15个菌株提取细胞DNA后克隆获得蒙古柳cDNA,在NCBI数据库中对测序得到的基因序列进行相似性比对,结果发现大部分和毛果杨基因有高的相似性,其中一些基因已有研究表明和盐胁迫相关。本研究可为挖掘可利用的耐盐基因资源提供依据。
In order to select Na Cl stress related genes of Salix linearistipularis, using FOX hunting system technology, double-stranded c DNA ligated into p YES2 vector and transformed into a yeast strains(In VSCI). The138 resistance yeast was isolated from p YES2-c DNA yeast in 1.0 mol/L Na Cl stress treatment, and further resistance after 1.0 mol/L NaCl, 15 yeast strains was obtained. The resistance of 15 strains was more higher than the control by NaHCO3, H2O2 and Srobitol stresses. After sequencing, these gene sequences had higher similarity with Populus trichocarpa, some of the genes were shown to salt stress related genes. This research can provide the basis for mining use of salt tolerant gene resources.
出处
《分子植物育种》
CAS
CSCD
北大核心
2015年第5期1123-1129,共7页
Molecular Plant Breeding
基金
中国教育部科研创新团队计划(IRT13053)资助