饲料虫草培养基水平对人工感染灿烂弧菌后刺参非特异性免疫指标及肠道菌群数量的影响

Effects of dietary cordyceps culture medium on non-specific immune parameters and intestinal microflora counts after artificially infected with Vibrio Splendidus of sea cucumber(Apostichopus Japonicus)

试验旨在研究饲料虫草培养基水平对人工感染灿烂弧菌后刺参非特异性免疫指标及肠道菌群数量的影响。试验配制虫草培养基(CMC)干粉水平分别为0%、5%、10%及15%的4组试验饲料,用以投喂体重(1.06±0.37)g的刺参。28 d养殖试验结束后,对刺参进行灿烂弧菌人工感染,分别于第0、1、3、5、7 d测定其体腔细胞吞噬率,体腔液酸性磷酸酶、碱性磷酸酶、溶菌酶、超氧化物歧化酶活性、肠道总菌数(TBC)及肠道弧菌数(VBC)数量的变化。结果表明:1刺参非特异性免疫指标随着人工感染灿烂弧菌时间的推移而改变,感染后第1至3 d各项指标相继下降,从第5 d开始恢复,至第7 d时几乎回到感染前水平;2感染后第7 d,投喂添加10%CMC饲料的刺参处理组,其非特异性免疫指标基本高于其它处理组;3人工感染灿烂弧菌后,刺参肠道TBC及VBC均有所升高,但各处理组间差异不显著(P>0.05)。综上所述,饲料中添加CMC能改善刺参体腔液非特异性免疫指标,增强其对灿烂弧菌的抵抗力,刺参饲料CMC适宜添加水平为10%。

A feeding trial was conducted to determine the effects of dietary cordyceps culture medium（CMC） after artificially infected with Vibrio splendidus of sea cucumber（Apostichopus japonicus）, focus-ing on non-specific immune parameters and intestinal microflora counts. Diets with four CMC levels（0, 5%, 10% and 15%） were fed to sea cucumber juveniles [（1.06±0.37） g]. After 28 day feeding trial,the sea cucumbers were injection-challenged with Vibrio splendidus and sampled on the 1st, 3rd, 5th and 7thday. The results showed that all of the coelomic fluid non-specific immune parameters changedover time. The non-specific immune parameters began to decrease on the 1stand 3rdday after infectionand recovered on 5thday. It almost recovered to the same level before infection on the 7thday. On the7 thday, the sea cucumbers fed diets with 10% CMC showed higher resistance to Vibrio splendidus byimproved the non-specific immune parameters. The intestinal TBC and VBC generally increased afterinfection, with no significant differences（P0.05） were observed. The results indicate that the optimumdietary CMC level for sea cucumber juveniles is 10%.