摘要
目的研究普罗布考对2型糖尿病动脉粥样硬化(As)的保护作用及可能机制。方法瘦素受体缺陷(db/db)与低密度脂蛋白受体敲除(LDLR-/-)杂交的2型糖尿病As易感模型小鼠(db/db-LDLR-/-),自8周龄开始喂饲含有0.5%普罗布考的高脂饲料,对照组为同样高脂饮食喂饲的db/db-LDLR-/-及单纯LDLR-/-小鼠。在实验2及4个月时分别检测各组小鼠血脂、血糖。实验4个月结束时,检测糖耐量及主动脉粥样硬化损伤情况。结果普罗布考能明显降低血浆总胆固醇(TC)、甘油三酯(TG)及血糖水平,显著改善受损的糖耐量,减低主动脉全长As斑块面积,同时血浆高密度脂蛋白胆固醇(HDLC)与TC比值(HDLC/TC)不变。结论普罗布考治疗对2型糖尿病As有明显的保护作用,可能成为临床治疗2型糖尿病大血管病变的有效药物。
Aim To evaluate the protective effects and possible mechanisms of probucol on atherosclerosis in hyperlipidemic type 2 diabetic mice.Methods The hyperlipidemic type 2 diabetic（db / db-LDLR-/-） mice were generated by the cross between leptin receptor deficient（db / db） mice and low density lipoprotein receptor knockout（LDLR-/-） mice.8-week-old db/db-LDLR-/-mice were fed with high fat diet（HFD） mixed with 0.5% probucol,diabetic（db / db-LDLR-/-） and non-diabetic（LDLR-/-） control mice were fed with the same HFD without probucol addition.At 2 and 4 months after HFD,plasma lipids and glucose levels were measured.At 4 months after HFD,glucose tolerance and ＂en face＂total aortic atherosclerotic plaque sizes were measured.Results Compared with type 2 diabetic HFD control group,probucol had strong protective effects on reducing plasma total cholesterol（TC）,triglyceride（TG）,glucose levels,improving glucose tolerance,and decreasing atherosclerotic lesions.Meanwhile,high density lipoprotein cholesterol（HDLC） / TC levels were unchanged.Conclusions Probucol had striking antiatherogenic effects under type 2 diabetic condition with hyperlipidemia.It might be an effective therapy in type 2 diabetic macrovascular disease.
出处
《中国动脉硬化杂志》
CAS
北大核心
2015年第7期668-672,共5页
Chinese Journal of Arteriosclerosis
基金
国家自然科学基金面上项目(81070242)
