血根碱在不同畜禽机体内残留量评定的研究

Study on sanguinarine residues in different animal's body

试验通过高效液相色谱-质谱联用法研究血根碱在鸡、鸭及猪肝脏、肾脏和肌肉中的残留状况,为血根碱预混剂作为植物源饲料添加剂使用的安全性提供依据与参考。采用单因素试验设计,试验一和试验二分别选取40日龄三黄鸡和60日龄攸县麻鸭各30只,分为对照组(基础饲粮)、高剂量组(基础饲粮+80 mg/kg血根碱预混剂)、低剂量组(基础饲粮+40 mg/kg血根碱预混剂),饲喂30 d后屠宰,取其肝脏、肾脏和肌肉样品,测定其中血根碱残留量;试验三选取20头80 kg左右健康杜×长×大三元杂交生长猪20头,分为对照组(基础饲粮)和试验组(基础饲粮+80 mg/kg血根碱预混剂),试验期为30 d,试验结束当天屠宰取其肝脏、肾脏和肌肉样品,测定其中血根碱残留量。试验一结果表明,高剂量组和低剂量组鸡的肝脏、肾脏和肌肉中血根碱残留量均低于定量限5 ng/g,对照组无血根碱残留;试验二结果显示,高剂量组和低剂量组的鸭肝脏、肾脏和肌肉中血根碱残留量均低于定量限5 ng/g,对照组无血根碱残留;试验三结果表明,试验组猪肝脏、肾脏及肌肉中血根碱残留量均小于定量限5 ng/g,对照组无血根碱残留。在本试验的血根碱添加量和测定方法条件下,鸡、鸭和猪脏器和肌肉中血根碱的残留量均低于5 ng/g。

The objective is to accumulate the basis data and reference about sanguinarine as an plantfeed additive, by testing safety test of the residual from liver, kidney and muscle with chicken, duckand pig, with the help of GC-MS spectrometry. Experiment 1： thirty 40-day chicken were divided intocontrol group（basal diet）, high group（basal diet＋80 mg/kg sanguinarine premix）, low group（basal diet＋40 mg/kg sanguinarine premix） by single factor test design. After 30 days experiment period, experimentanmials were slaughtered, the liver, kidney and muscle samples were used to test sanguinarine residues.Experiment 2： select thirty 60-day Youxian ducks instead of chicken, others was follow experiment 1.Experiment 3： Twenty 80 kg Duroc × Landrace × Largewhite were divided into control group（basal diet）and experimental group（basal diet＋80 mg/kg sanguinarine premix agent） by random, the test period was30 days. Liver, kidney and muscle samples were used to test sanguinarine residues. Experiment 1 resultsshowed that sanguinarine residues of chicken＇s liver, kidney and muscle were lower than 5 ng/g, which isthe lowest residues standard, in the high experiment group and low experiment group. Control group hadno residues. Experiment 2＇s and experuiment 3＇sresults were as the same as experiment 1. Sanguin-arine residues of the liver, kidney and muscle inchicken, duck and pig were lower than 5 ng/g fol-lowing this adding amount and this test method.