REV3基因rs12211763、rs465646与宁夏汉族人群结直肠癌的关联性研究

The Association between rs12211763,rs465646 of REV3 Genes and Colorectal Cancer in Han Population of Ningxia

目的探讨REV3基因rs12211763和rs465646与宁夏汉族人群结直肠癌(colorectal cancer,CRC)易感性的关系。方法采用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)方法对204例CRC患者和207例对照的REV3基因rs12211763和rs465646进行基因分型,并通过SHEsis在线软件构建单倍型。结果 rs12211763、rs465646的基因型和等位基因频率在病例组和对照组间无统计学差异(P>0.05)。按肿瘤发生部位进行分层分析后,两位点的基因型频率在结肠癌组和对照组间、直肠癌组和对照组间的差异均无统计学差异(P>0.05)。rs12211763、rs465646的单倍型频率在病例组和对照组间无统计学差异(P>0.05)。结论 REV3基因的rs12211763和rs465646可能与宁夏汉族人群结直肠癌的遗传易感性无关。

Objective to investigate the correlation between rs12211763,rs465646 of REV3 gene and susceptibility of colorectal cancer（ CRC） in Han population of Ningxia. Methods rs12211763 and rs465646 of REV3 gene were genotyped by Polymerase Chain Reaction- Restriction Fragment Length Polymorphism（ PCR- RFLP） method in 204 CRCs and 207 controls,and the haplotypes were created by SHEsis online software. Results Genotype and allele frequency distribution of rs12211763,rs465646 between cases and controls had no statistically significant difference（ P〉0. 05）. After stratification from the cancer location,the genotype frequency distributions of two polymorphisms,colon cancer and rectal cancer patients with that in controls,had no statistically significant difference（ P〉0. 05）. The haplotype distributions derived from rs12211763 and rs465646 didn＇t have significant difference between the two groups（ P〉0. 05）. Conclusion rs12211763 and rs465646 of REV3 may have no relationship with the susceptibility of CRC.