抗BRAF抗体检测在类风湿关节炎诊断中的应用

The Clinical Application of Anti- BRAF Antibody in the Diagnosis of Rheumatoid Arthritis

目的检测类风湿关节炎(rheumatoid arthritis,RA)患者血清中抗鼠科肉瘤病毒癌基因同源物B1(vraf murine sarcomaviral oncogene homologue B1,BRAF)抗体水平并探讨其在RA诊断的临床意义。方法应用ELISA检测90例RA患者,71例其他风湿性疾病患者和18例正常对照组血清中抗BRAF抗体水平,分析RA患者血清抗BRAF抗体水平与临床和实验室其它指标的相关性。结果 1RA患者血清中抗BRAF抗体水平明显高于其他风湿性疾病组及正常对照组(P<0.05),而其他风湿性疾病组与正常对照组间抗BRAF抗体水平差异无统计学意义(P>0.05)。2RA组中类风湿因子(rheumatoid factor,RF)、抗环瓜氨酸肽(cyclic citrullinated pepfide,CCP)抗体均阴性患者与RF或抗CCP抗体阳性患者血清抗BRAF抗体水平差异无统计学意义(P>0.05)。RA组中抗角蛋白抗体(antikeratin antibody,AKA)阳性组与AKA阴性组血清抗BRAF抗体水平差异无统计学意义(P>0.05)。3RA组中抗BRAF抗体水平与RF、病程、血红蛋白(HGB)呈负相关。RA组中抗BRAF抗体水平与年龄、红细胞沉降率、C反应蛋白、关节肿胀指数、关节疼痛指数无明显相关性。结论抗BRAF抗体检测可用于RA患者的诊断,并与病情活动程度有一定相关性。

Objective To detect the serum levels of the antibodyagainst v raf murine sarcoma viral oncogene homologue B1（ BRAF） and to assess its clinical significance in the diagnostic practice of rheumatoid arthritis（ RA）. Methods The serum anti- BRAF antibody level in 90 cases of patients with RA and 71 patients with other rheumatic disease patients and 18 normal controls were detected by enzyme- linked immunosorbent assay（ ELISA）. Mann- Whitney U test and Spearman correlation analysis were used for Statistical analysis. Results The anti- BRAF antibody levels were significantly higher in the patients of RA than in other rheumatic diseases and normal control group（ P〈0. 05）. The anti- BRAF antibody levels had no significant differences between other rheumatic diseases and normal control group（ P〉0. 05）. ② There was not statistically significance in the group of patients with betwent of rheumatoid factor（ RF）,anti- cyclic citrullinated peptide（ CCP） antibodies negative and RF or anti- CCP antibody- positive（ P〉0. 05）. There was not statistically significance in the group of patients with betwent of antikeratin antibody negative and antikeratin antibody positive（ P〉0. 05）.③ The levels of anti- BRAF antibody was negative correlation with RF,duration,hemoglobin（ HGB） in the group of RA. The levels of anti- BRAF antibody were no significant correlation with age,erythrocyte sedimentation rate,C- reactive protein,joint swelling index,joint pain index in the group of RA.Conclusion The detection of anti- BRAF antibodies can be used for the diagnosis of RA and has some relevance with the severity of disease activity.