人退变颈椎间盘髓核组织Caspase-1、IL-1β表达与细胞凋亡的关系

Relationship between Apoptosis and Related Gene Expressions of Caspase- 1and IL- 1β in Degenerated Human Cervical Intervertebral Discs

目的检测人退变颈椎间盘髓核组织的细胞凋亡率以及相关蛋白Caspase-1及IL-1β的表达。方法将30例颈椎间盘突出症患者(实验组)的间盘髓核组织和30例颈椎骨折患者(对照组)的间盘髓核组织,采用原位DNA片段末端标记(TUNEL)法和免疫组织化学方法,观察其细胞凋亡状态及Caspase-1及IL-1β的表达。结果实验组TUNEL阳性细胞率为(10.21±1.13)%,高于对照组的(6.34±1.65)%(P<0.01);实验组Caspase-1、IL-1β蛋白阳性细胞率分别为(42.31±8.08)%、(56.07±10.22)%,高于对照组的(22.06±1.28)%、(43.12±13.56)%(P<0.01)。将实验组进行Pearson相关分析,发现间盘髓核组织TUNEL阳性细胞率和Caspase-1、IL-1β阳性细胞率呈正相关,相关系数r分别为0.828和0.780(P<0.01)。结论在人退变颈椎间盘髓核中,高表达的Caspase-1、IL-1β与其细胞凋亡有关,是引起盘源性颈椎间盘退变的原因之一。

Objective To detect the apoptosis rates of degenerated human intervertebral disc cartilage endplate and nucleus pulposus cells and the related gene expressions of Caspase- 1 and IL^-1β. Methods The apoptosis state and expressions of Caspase- 1 and IL^-1β in the disc tissues of 30 patients with cervical spondylosis and 30 patients with cervical trauma in the experimental group were observed by using Td T- mediated d UTP Nick End Labeling（ TUNEL） of DNA fragments and immunohistochemical method. Results In the nucleuspulposustissue,therateofTUNEL-positivecellswas（10.21±1.13）%）intheexperimentalgroupanditwashigherthan（6.34±1.65）%inthecontrolgroup（P0. 01）. The rates of Caspase- 1 and IL^-1β proteins- positive cells were（ 42. 31 ± 8. 08） % and（ 56. 07 ±10. 22） % in the experimental group and they were higher than（ 22. 06 ± 1. 28） % and（ 43. 12 ± 13. 56） % in the control group（ P〈0. 01）. Pearson correlation analysis in experimental group showed disc nucleus pulposus TUNEL positive cells and Caspase- 1,IL^-1β- positive cells was positively correlated（ the correlation coefficient r = 0. 828 and 0. 780,P〈0. 01）. Conclusion In degenerated human intervertebral disc cartilage endplate and nucleus pulposus tissue,highly expressed Caspase- 1 and IL^-1β are involved in the regulation of apoptosis,and this is the cause of discogenic cervical disc degeneration.