TLR9激动剂CpG-ODN对HepG2肝癌细胞增殖及侵袭的影响

The Influence of the Activator Cp G- ODN of TLR9 on the Proliferation and Invasion of Hepatocellular Cancer Cells Hep G2

目的探讨Toll样受体9(TLR9)激动剂未甲基化的胞嘧啶-鸟嘌呤核苷酸序列(Cp G-ODN)对Hep G2肝癌细胞增殖及侵袭的影响。方法应用不同浓度的Cp G-ODN作用于肝癌细胞株Hep G2不同时间,采用实时荧光定量PCR法检测Hep G2中TLR9mRNA表达情况,MTT法检测Hep G2细胞的增殖变化,Transwell法检测其侵袭能力变化。结果 Cp G-ODN组(4、16μg·m L-1)与阴性对照组比较可增加Hep G2细胞中TLR9mRNA的表达;Cp G-ODN(1、4、16μg·m L-1)激动TLR9后可促进Hep G2细胞增殖,且以48h最为显著,并存在剂量依赖关系;Cp G-ODN(4、16μg·m L-1)激动TLR9可增强Hep G2细胞的侵袭能力(P均<0.05)。结论 TLR9表达上调可促进肝癌细胞增殖和侵袭。

Objective To investigate the influence of the activator Cp G- ODN of TLR9 on the proliferation and invasion of hepatocellular cancer cells Hep G2. Methods Cp G- ODN with different concentration was administered to the hepatocellular cancer cells Hep G2. The expression of TLR9 mRNA was detected by real-time PCR and the proliferation of Hep G2 cells were determinded by MTT assay while the changes of invasion ablity were monitored by transwell assay. Results Cp G- ODN（ 4,16μg·m L^-1） could significantly increase the TLR9 mRNA expression level compared with the negative control group. The proliferation of Hep G2 cells could be promoted when TLR9 was activated by Cp G- ODN（ 1,4,16μg·m L^-1） and the most obvious change was at the 48 h point,which was a dose- dependent manne. The invasion ability of Cp G- ODN（ 4,16μg·m L^-1） was significantly enhanced by TLR9（ P〈0. 05）. Conclusion Upregulation of TLR9 can promote proliferation and invasion in hepatocellular cancer cells.