正交设计优选茉莉花叶总黄酮提取工艺研究

Extraction Technology Research on Orthogonal Excogitation to Optimise the Total Flavonoids of Jasmine's Leaves

目的:研究茉莉花叶总黄酮提取工艺的优化。方法:采用乙醇浸提法提取总黄酮,考察乙醇体积分数、料液比、温度、提取时间四个因素的影响,并在波长358 nm处测定茉莉花叶总黄酮的量,采用(33)正交试验法进行优选提取工艺。结果:茉莉花叶总黄酮最佳提取工艺为每次提取2.5 h,提取温度70℃,料液比1:60(g/ml),乙醇体积分数60%,产率约为1.907%。结论:试验结果表明优选出的提取工艺产率较高、方便可靠。

Objective： Research on Extraction technology to optimise the total flavonoids of Jasmine leaves. Methods： Extract total flavonoids through ethanol digestion,observe the influence of four factors about ethanol volume fraction,solid to liquid ratio,temperature and time,measure the weight of jasmine flavonoids in 358 nm,use（ 33） orthogonal test to optimize the extraction process. Results： Optimum process to extract the total flavonoids of Jasmine leaves is extracting 2. 5 hours,70 ℃,1∶ 60（ g/ml） solid to liquid ratio and 60%ethanol volume fraction each time,the productivity is 1. 907%. Conclusion： The experimental results show that the optimized extraction process is more convenient and reliable.