水线草和白花蛇舌草HPLC特征图谱研究

Comparative Study on HPLC Specific Chromatogram of Oldenlandia corymbosa Linn. And Oldenlan diadiffusa( Willd.) Roxb

目的建立不同产地中药材水线草与白花蛇舌草的HPLC特征图谱,用以区分水线草与白花蛇舌草。方法采用HPLC分析,色谱柱为Agilent Eclipse XDB-C_(18)(4.6 mm×250 mm,5μm),以甲醇-水为流动相系统进行梯度洗脱,流速1.0 m L·min^(-1),检测波长254 nm,柱温30℃;采用《中药指纹图谱相似度评价系统》进行相似度评价;采用Matlab7.0软件进行主成分分析和聚类分析。结果分别得到水线草和白花蛇舌草的HPLC特征图谱,经比较,两者共有峰为8个,分别为a^h;特征峰水线草中峰为7~12,白花蛇舌草中峰为1~6;相似度分析结果显示,不同来源水线草相似度值在0.733~0.984之间,不同来源白花蛇舌草相似度值在0.873~0.951之间;主成分分析和聚类分析将供试药材分为3类:自采水线草、自采白花蛇舌草及购买白花蛇舌草。结论该方法可以快速简便地区分中药材水线草和白花蛇舌草,可为两者的质量控制及临床使用提供参考。

OBJECTIVE To establish the HPLC specific chromatogram of Oldenlandia corymbosa Linn. and Oldenlan diadiffusa （Willd.） Roxb. in different regions so as to distinguish these two traditional Chinese medicinal materials. METHODS HPLC was performed on an Agilent Eclipse XDB-C18 （4. 6 mm ×250 mm ,5μm） column with mobile phase consisting of methanol-water at a flow rate of 1 mL min-1, column temperature at 30℃ and detection wavelength at 254 nm. Similarity evaluation system for chromato- graphic fingerprint of TCM was used to conduct similarity evaluation and Matlab7.0 software was used for principal component and clus- ter analysis. RESULTS Oldenlandia corymbosa Linn. and Oldenlan diadiffusa （Willd.） Roxb. had eight common peaks, namely, a -h; the characteristic peaks of 0. corymbosa were 7 to 12, while the characteristic peaks of O. diffusa were 1 to 6. The similarity values of O. corymbosa and O. diffusa collected from different sources were 0. 733 -0. 984 and 0. 873 -0. 951, respectively. Accord- ing to the principal components analysis and cluster analysis, the tested samples were classified into three categories： O. corymbosa collected from wild, O. diffusa collected from wild and purchased 0. diffusa. CONCLUSION The established method is reliable and rapid to distinguish the two kinds of easily confused traditional Chinese medicinal materials and also can offer reference for their quality control and clinical use.