基于基线等比增减设计法优选黄芪多糖-皂苷的免疫调节最佳配比

Optimization of the best ratio of Astragalus polysaccharide and Astragalus saponin for immunomodulation based on increase-decrease baseline geometric proportion design method

目的:考察黄芪多糖、黄芪皂苷配伍应用对环磷酰胺模型小鼠的免疫调节活性,筛选最优配比。方法:采用环磷酰胺(40 mg·kg^(-1))制造免疫抑制小鼠模型,制备脾淋巴细胞悬液,分别进行正常培养和刀豆蛋白诱导培养,同时给予黄芪多糖与皂苷不同配比药液(10∶0,4∶1,3∶2,1∶4,0∶10),作用48 h后行MTT微量比色法检测OD值;采用该模型,连续5 d灌胃给予相应受试药液,检测各药物组对模型鼠血清TNF-α含量、脾脏指数、胸腺指数的影响。结果:糖-苷10∶0组、4∶1组、3∶2组均可促进体外模型鼠正常培养的脾淋巴细胞增殖(P<0.01),以4∶1组作用显著,各配比组均抑制体外模型鼠经ConA诱导培养的脾淋巴细胞增殖,以糖-苷4∶1组抑制最弱;与模型组比较,灌胃给药后除0∶10组外,各组均能明显提高模型小鼠血清TNF-α含量(P<0.05或P<0.01),亦以4∶1组作用显著,且4∶1组、3∶2组对脾脏指数有明显提高作用(P<0.05),而对胸腺指数各配比组均无显著影响(P>0.05)。结论:黄芪多糖与皂苷不同比例配伍对免疫抑制模型小鼠均表现出不同程度的免疫调节作用,尤以糖-苷4∶1组作用最强,为最佳配比。

OBJECTIVE To study the immunomodulating effects of different proportions of astragalus polysaccharide（APS）and astragalus saponin（AS）on immunosuppressed mice induced by cyclophosphamide（CTX）.METHODS Immunosuppressed mouse models were established by using CTX,spleen cell suspension was prepared to perform normal cultivation and canavaline induction cultivation.Drug solution with different APS：AS ratios（10∶0,4∶1,3∶2,1∶4,0∶10）were given in vitro for 48 hours to determine OD value by using MTT microcolorimetry.Corresponding drug solutions were gavaged for 5 days consecutively by using this model to determine the influence of different groups on serum TNF-αcontent,spleen and thymus index.RESULTS Compared with model control group,APS-AS 10∶0,4∶1,3∶2 groups all could promote lymphocyte proliferation in vitro（P〈0.01）,and the group with 4∶1 APS-AS had more obvious effects than other groups.Compared with model control group,APS-AS 10∶0,4∶1,3∶2,1∶4 groups all could evidently increase the level of TNF-αin serum（P〈0.05,P〈0.01）,and APS-AS 4∶1,3∶2 groups could raise spleen index（P〈0.05）.CONCLUSION Different proportions of APS and AS have immunomodulating effects of varied degrees on CTX induced immunosuppressed mice.The group with 4∶1 APS-AS has the best effects.