樟子松树皮多酚的成分分析和结构鉴定

Composition analysis and structural identification of polyphenols from Pinus sylvestris var. mongolica barks

本文采用紫外吸收光谱、红外光谱、高效液相色谱和液质联用方法对樟子松树皮中多酚化合物进行成分分析和结构鉴定。结果表明：通过UV-VIS分析松多酚在240~280、300~400 nm这2个波长范围内均有吸收峰,推断松多酚中含有黄酮类物质。通过IR分析可以得到松多酚的酚羟基Ar—OH在3 400 cm-1νO—H对称伸缩振动产生强而宽的特征吸收峰以及在1 390~1 315 cm-1的羟基δO—H面内弯曲振动吸收峰,说明松多酚分子中存在酚羟基,属于酚类物质。经HPLC和LC-MS分析可以推断樟子松多酚中主要有7种组成成分,分别为儿茶素、芦丁、绿原酸、没食子酸、芥子酸-葡糖苷、芍药-3-葡萄糖（半乳糖）糖苷或者矢车菊-3-（6-O-乙酰基）-葡糖（半乳糖）糖苷、芥子酸芍药色素-3-槐糖苷。

The composition of polyphenols from Pinus sylvestris L. var. mongolica were analyzed and their structures were identified with UV-VIS,IR,HPLC and LC-MS. The UV-VIS results showed that there are absorption peaks in regions of 240-280 nm and 300-400 nm,indicating that flavonoids exist in the pine polyphenols. Fourier Transform Infrared Spectrum analysis showed that a strong and broad absorption peak at 3 400 cm-1is generated from stretching and vibration of phenolic hydroxyl Ar-OH and absorption peaks at 1 390-1 315 cm-1due to characteristics of in-plane bending vibration of hydroxyl δO—H,which indicated that hydroxyl groups existed in the pine polyphenols. Combined application of HPLC and LCMS showed that there are seven components in P. sylvestris L. var. mongolica polyphenols,i. e.,catechuic acid,rutin,chlorogenic acid,gallic acid,sinapic acid-dextrose glucoside,peony-3-glucoside or centaurea-3-（ 6-o-acetyl）-glucose glycoside,and sinapic acid peonidin-3-sophoroside composition.