不同灸温对高脂血症小鼠胆固醇及“神阙”穴皮肤的影响

Effects of different moxibustion temperature on cholesterol and skin around “Shenque”(CV 8)in mice with hyperlipidemia

目的:通过观察不同温度艾灸对急性高脂血症模型小鼠胆固醇及"神阙"穴皮肤的影响,探讨艾灸效应与瞬时感受器电位香草酸受体1(transient receptor potential vanilloid 1,TRPV1)的相关性。方法:32只C57BL/6J野生型(WT)小鼠选取8只作为空白组,余24只采用注蛋黄乳法制备急性高脂血症模型,造模成功后随机分为模型组、38℃艾灸组、46℃艾灸组,每组8只。38℃艾灸组施灸温度控制在(38±1)℃,46℃艾灸组施灸温度控制在(46±1)℃,两组均取"神阙"和双"足三里",每穴每次艾灸10min,每日1次,共治疗2次。空白组和模型组以同样方法抓取固定,不予艾灸治疗。治疗结束后,采用氧化酶法检测血清胆固醇含量;HE染色观察各组小鼠"神阙"穴皮肤组织形态;免疫组化法检测皮肤组织内TRPV1的表达。结果:1与空白组比较,模型组血清胆固醇含量升高(P<0.001);46℃艾灸组血清胆固醇含量较模型组降低(P<0.001);46℃艾灸组血清胆固醇含量较38℃艾灸组降低(P<0.01)。238℃艾灸组皮肤组织形态结构有轻微的改变;46℃艾灸组皮肤组织形态结构改变明显,出现了特异性的变化。346℃艾灸组"神阙"穴皮肤组织内TRPV1表达与空白组、模型组比较差异均有统计学意义(均P<0.01),与38℃艾灸组比较差异亦有统计学意义(P<0.05)。结论:温度是影响小鼠胆固醇和穴位皮肤的重要因素,艾灸效应与TRPV1有相关性。

Objective To observe the effects of moxibustion with different temperatures on cholesterol and skin around＂Shenque＂（CV 8）in mice with acute hyperlipidemia,and to explore the correlation between moxibustion effect and transient receptor potential vanilloid 1（TRPV1）.Methods Eight mice among 32 mice of C57BL/6J wild type（WT）were selected into a blank group,and the remaining 24 mice were made into the acute hyperlipidemia model by injection of egg yolk.After model establishment,the mice were randomly divided into a model group,a 38 ℃ moxibustion group and a 46 ℃ moxibustion group,8 mice in each group.The temperature was（38±1）℃in the 38 ℃ moxibustion group and（46±1）℃in the 46 ℃ moxibustion group.＂Shenque＂（CV 8）and ＂Zusanli＂（ST 36）were selected in the two groups;moxibustion was given for 10 min per time,once a day for2 times.Mice in the blank group and model group were treated with immobilization and moxibustion was not given.After treatment,the level of serum cholesterol was tested by oxidase method;the morphology of skin around＂Shenque＂（CV 8）was observed by HE staining;the expression of TRPV1 in skin was measured by immunohistochemistry.Results 1 Compared with the blank group,the level of serum cholesterol was increased in the model group（P〈0.001）;compared with the model group,the level of serum cholesterol was reduced in the 46℃ moxibustion group（P〈0.001）;compared with the 38 ℃ moxibustion group,the level of serum cholesterol was reduced in the 46 ℃ moxibustion group（P〈0.01）.2 There was slight change of morphology and structure in skin tissue in the 38℃ moxibustion group,while obvious change was observed in the 46 ℃ moxibustion group,indicating specific change.3 The difference of the expression of TRPV1 in skin was significantly different between 46 ℃ moxibustion group and blank group,model group（both P〈0.01）;it was also different from the38 ℃ moxibustion group（P〈0.05）.Conclusion Moxibustion temperature is one of the important factors affecting the cholesterol and acupoint skin.The effects of moxibustion are related to TRPV1.