摘要
应用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)方法对三叶赤楠、火棘、三角枫、鸡爪槭和金边瑞香这5种江西特色盆景树种根际微生物特征进行了比较研究。结果表明:PCR-DGGE对于盆景树种根际微生物特征响应良好,DGGE图谱可以明显反映出5种盆景树种根际微生物的差异。不同树种根际微生物特征有很大区别,鸡爪槭、三角枫根际微生物多样性指数最高,为3.121和2.876,并且根际微生物群落相似程度很高;而火棘根际微生物多样性指数最低,为1.038。这种根际微生物群落的差异可能是由不同盆景植物的根系分泌物差异所造成的,鸡爪槭、三角枫根际微生物群落相似可能是由于其系统发育地位较近(槭树科槭属)所造成的。而火棘(蔷薇科)根际微生物多样性较低可能是由于蔷薇科植物根系分泌物富含三萜酸类物质,对微生物具有抑制作用的原因。
The polymerase chain reaction- denaturing gradient gel electrophoresis（ PCR- DGGE）method was used to determine the rhizosphere microorganism diversity of five bonsai species including Syzygium grijsii, Pyracantha fortuneana, Acer buergerianum, Acer palmatum, and Daphne odora. The results showed that the rhizosphere microorganism had significant differences among the five bonsai species. Electrophoresis lanes obtained by PCR - DGGE were able to reflect the difference. Soil microorganism diversity were higher in Acer palmatum and Acer palmatum, with peak diversity index of 3. 121 and 2. 876, they also had a higher similarity of rhizosphere microbial community. The lowest diversity index was observed in Pyracantha fortuneana, and the value was 1. 038. These differences in rhizosphere microbial communities may be caused by root exudate secreted from different bonsai plants. Higher similarity of rhizosphere microbial community from Acer palmatum and Acer palmatum may be due to their close phylogenetic position（ Aceraceae, Acer）. A lower rhizosphere microbial diversity in Pyracanthafortuneana may be due to the root exudate secreted by Rosaceae plant, which contained substances triterpene acids and had inhibitory effect on microorganism.
出处
《森林工程》
2016年第1期33-36,53,共5页
Forest Engineering
基金
国家自然科学基金(31400528)
江西省自然科学基金(20151BAB214018)资助
