利用体外抑菌试验确立奶牛子宫内膜炎抗菌蒙兽药组方的研究

Study on the establishment of antibacterial composing prescription of Mongolian veterinary drugs against endometritis in dairy cows using bacteriostic test in vitro

为了筛选出治疗奶牛子宫内膜炎的最佳抑菌效果的蒙兽药组方,试验针对从奶牛子宫内膜炎分泌物中分离出的强致病性大肠埃希氏杆菌和金黄色葡萄球菌,采用牛津杯法对26味蒙兽药单药进行体外抑菌试验,筛选出对这两种致病菌均敏感的10味蒙兽药。根据药物抑菌浓度试验选取其中无相互颉颃作用的5味蒙兽药,通过SPSS软件进行正交试验设计,并结合体外抑菌试验,筛选出最佳的抑菌药物组合,再利用SPSS正交试验优化用药剂量。结果表明:结合正交试验和体外抑菌试验所确立的抗菌蒙兽药组方的抗菌效果显著强于各味单药的抑菌效果,且与环丙沙星药敏纸片的效果相当。说明筛选出的蒙兽药组方对治疗子宫内膜炎是可行的。

To screen a composing prescription of Mongolian veterinary drugs with the best bacteriostic effect for the treatment of endometritis in dairy cows, strongly pathogenic Escherichia coli and Staphylococcus aureus strains were isolated from secretions of endometritis in dairy cows. The 26 kinds of single Mongolian veterinary drugs were used for a bacteriestic test in vitro using the Oxford cup method, and then 10 kinds of the Mongolian veterinary drugs were screened, which were sensitive to the two kinds of pathogenic bacteria. Five kinds of Mongolian veterinary drugs were selected according to the drug inhibitory concentration, which had no mutual antagonistic effect. An orthoganal design was conducted by SPSS software, combined with the bacteriostic test in vitro, the best combination of bacteriostie drugs was selected. The orthogonal design conducted by SPSS software was used for the optimization of the dosage of drugs again. The results showed that the antibacterial effect of anti- bacterial composing prescription of Mongolian veterinary drugs established through combining with the orthogonal experimental design and antibacterial test in vitro, was significantly stronger than the inhibitory effect of each single drug, and the antibacterial effect of the composing prescription was equal to that of ciprofloxacin susceptibility disks. The result indicates that the screened composing proscription of Mongolian veterinary drugs is feasible to treat endomctritis in daii＇y cows.