司替戊醇对映异构体的HPLC法拆分及其含量测定

Chiral Separation and Determination of Stiripentol Enantiomers by HPLC

建立了高效液相色谱法拆分并测定司替戊醇对映异构体。采用Chiralpak AY-H色谱柱,以正己烷∶异丙醇（90∶10）为流动相,检测波长270 nm。（R）、（S）-司替戊醇均在15.6-500？g/ml范围内线性关系良好。平均回收率为98.60%和99.84%,RSD为0.82%和0.72%。

An HPLC method was established for the enantiomeric separation and determination of stiripentol enantiomers. A Chiralpak AY-H column was used, with the mobile phase of n-hexane ： isopropanol （90 ： 10）, at the detection wavelength of 270 nm. It was linear in the concentration range of 15.6-500 μg/ml for both （R）- and （S）- stiripentol. Their recoveries were 98.60 % and 99.84 %, with RSDs of 0.82 % and 0.72 %.