葡萄糖对铜绿假单胞菌生物膜形成能力的影响

Impact of glucose on Pseudomonas aeruginosa biofilm formation ability

目的探索不同浓度的葡萄糖对铜绿假单胞菌生物膜形成能力的影响。方法用含有不同葡萄糖浓度的胰蛋白胨大豆肉汤培养铜绿假单胞菌,测定其570 nm处光密度,通过函数Linest进行线性回归拟合,用激光扫描共聚焦显微镜(CLSM)观察不同浓度葡萄糖培养铜绿假单胞菌生物膜的形成过程及厚度。结果 22株细菌不同浓度葡萄糖的A_(570)拟合直线的斜率均大于0,其中4株菌上升趋势明显。与无糖组及低浓度葡萄糖组相比,当葡萄糖浓度≥11.1mmol·L^(-1)时,生物膜成熟速度加快、成熟时间减少、粘附能力增强、厚度增加(P<0.05)。结论葡萄糖浓度≥11.1 mmol·L^(-1)时对铜绿假单胞菌生物膜形成有明显的诱导作用,并且随葡萄糖浓度的升高,诱导作用增强。

Objective To explore the impact of different concentrations of glucose on the ability of Pseudomonas aeruginosa biofilm formation.Methods The A_（570） of Pseudomonas aeruginosa cultured under different concentrations of glucose was measured and linear was regressed by function Linest. The formation was observed and the thickness was recorded by a laser scanning confocal microscope（ CLSM）. Results Experiments showed that with the increasing concentration of glucose in the culture medium,the ability of 22 bacteria biofilm formation showed raising trend（ all of the slope of the analogy line were positive）. Among them,4strains had apparently raising trend. CLSM images demonstrated that compared with no glucose group and lower concentration glucose group,there showed faster speed,less mature time,stronger adhesion ability and thicker thickness of biofilms in the group with glucose concentration was ≥11. 1 mmol · L^（-1）（ P〈0. 05）. Conclusion When the glucose concentration was ≥11. 1 mmol·L^（-1）,for Pseudomonas aeruginosa,the ability of biofilm formation was significantly induced and enhanced with the increasing concentration of glucose.