Rho激酶在醛固酮诱导的血管平滑肌细胞增殖中的作用

Role of Rho kinase in vascular smooth muscle cell proliferation induced by aldosterone

目的研究Rho激酶通路对醛固酮(ALD)诱导的血管平滑肌细胞(VSMC)增殖的影响。方法用Western blot法检测ALD诱导的血管平滑肌细胞Rho激酶的表达,氚标记胸腺嘧啶掺入法检测ALD诱导的VSMC的增殖,并观察ALD受体MR在此过程中的作用。结果 ALD可剂量、时间依赖地上调VSMC Rho激酶的表达,最强的刺激时间为20 min,最强的刺激浓度为100nmol·L^(-1),ALD受体拮抗剂——依普利酮可显著抑制ALD诱导的VSMC Rho激酶的上调及VSMC的增殖,依普利酮和Rho激酶抑制剂——Y-27632可显著逆转ALD的上述效应。结论 Rho激酶信号传导通路及ALD受体在ALD诱导的VSMC增殖中起重要作用。

Objective To explore the role of Rho kinase in vascular smooth muscle cell（ VSMC） proliferation induced by aldosterone（ ALD）and the effect of mineralcocorticoid receptor（ MR）. Methods The expressions of Rho-kinase protein were detected by Western blot. VSMC proliferation was measured by [3H] Thymidine incorporation. Results ALD could up-regulate the expressions of Rho-kinase protein in dose-and time-dependent manner,peak at 20 min and 100 nmol·L^-1Eplerenone,mineralcocorticoid receptor antagonist,could significantly reverse these effects,indicating the activation of Rho-kinase pathway induced by ALD in VSMC was mediated by MR. ALD could significantly induce VSMC proliferation,which could be inhibited by eplerenone and Y-276329（ Rho-kinase inhibitor）,indicating the VSMC proliferation induced by ALD was mediated by MR and the activation of Rho-kinase pathway. Conclusion Rho-kinase pathway and MR play an important role in VSMC proliferation induced by ALD.