小儿泌尿外科长期置管患儿病原菌分布与对Smad通路蛋白的影响研究

Distribution of pathogens isolated from long-term catheterization children of pediatric urology department and impact on serum Smad protein

目的对小儿泌尿外科长期置管患儿病原菌分布及对Smad通路蛋白的影响进行研究,为临床治疗提供参考。方法对医院2010年3月-2014年3月诊治的120例小儿泌尿外科长期置管患儿的临床资料进行分析,应用全自动微生物鉴定仪对病原菌进行鉴定,以同期健康体检儿童260名为对照组,小儿泌尿外科长期置管后未感染患儿260例为未感染组,酶联免疫吸附测定法分析血清中Smad1、Smad2和Smad3蛋白的表达;采用WHONE5.5软件和SPSS19.0软件对数据进行统计分析。结果感染患儿共分离出病原菌120株,其中革兰阳性菌65株占54.2%,以表皮葡萄球菌、G型溶血型链球菌和酿脓链球菌为主,革兰阴性菌55株占45.8%,以阴沟肠杆菌和铜绿假单胞菌为主;小儿泌尿外科长期置管患儿感染后引起血清中Smad1、Smad2和Smad3蛋白表达升高,且感染组患儿的表达明显高于对照组和未感染组患儿,差异均有统计学意义(P<0.05);小儿泌尿外科长期置管感染后引起患儿血清中TGFβ、VEGF及白细胞介素1蛋白表达升高,且感染组患儿的表达明显高于对照组和未感染组患儿,差异均有统计学意义(P<0.05)。结论小儿泌尿外科长期置管患儿容易受到病原菌感染,且患儿感染后血清中TGFβ-Smad通路蛋白表达明显升高。

OBJECTIVE To investigate the distribution of pathogens isolated from long-term catheterization children of pediatric urology department and observe the impact on serum Smad protein so as to provide guidance for clinical treatment.METHODS The clinical data of 120long-term catheterization children who were treated in the pediatric urology department from Mar 2010 to Mar 2014 were analyzed,and the pathogens were identified by using automatic microorganism identification system;260healthy children who receive physical examination were chosen as the control group,and 260long-term catheterization children without infections were set as the non-infection group.The expressions of serum Smad1,Smad2,Smad3 proteins were analyzed by means of enzyme-linked immunosorbent assay,and the statistical analysis of data was performed with the use of WHONE5.5software and SPSS19.0software.RESULTS A total of 120 strains of pathogens were isolated from the children with infections,including 65（54.2%）strains of gram-positive bacteria and 55 strains of gram-negative bacteria;the Staphylococcus epidermidis,Streptococcus hemolyticus G,and Streptococcus pyogenes were the major species of the grampositive bacteria;the Enterobacter cloacae and Pseudomonas aeruginosa were dominant among the gram-negative bacteria.The levels of serum Smad1,Smad2,and Smad3 proteins of the long-term catheterization children were elevated after they had the infections and were significantly higher in the infection group than in the control group and the non-infection group（P〈0.05）.The levels of serum TGFβ,VEGF,and interleukin 1of the long-term catheterization children were elevated after they had the infections and were significantly higher in the infection group than in the control group and the non-infection group（P〈0.05）.CONCLUSIONThe long-term catheterization children of the pediatric urology department are vulnerable to the infections,and the level of serum TGFβ-Smad signaling pathway protein is remarkably elevated after the children have the infections.