摘要
目的:建立HPLC法同时测定扶正平消胶囊中龙胆苦苷和迷迭香酸的含量。方法:采用Agilent Eclipse XDB-C18色谱柱(250mm×4.6mm,5μm),以0.1%甲酸(A)-乙腈(B)为流动相进行梯度洗脱,0-5min 88%A,5-10min88%-82%A,10-15min 82%-70%A,15-20min 70%-60%A;流速1.0ml/min;柱温25℃;检测波长280nm。结果:龙胆苦苷在6.75-431.6μg/ml(r=1.000),迷迭香酸在3.16-201.8μg/ml范围内呈良好线性(r=1.000)。日内和日间精密度均〈2%(n=3)。平均加样回收率分别为(100.89±2.25)%和(98.19±1.13)%(n=6)。结论:该方法简便、结果准确、重复性好,可用于扶正平消胶囊中龙胆苦苷和迷迭香酸的质量控制。
Objective:To establish a HPLC method for simultaneous determination of gentiopicroside and rosmarinic acid in Fuzhengpingxiao capsules.Methods:Agilent Eclipse XDB-C18column(250mm×4.6mm,5μm)was used for separation.Mobile phase was composed of 0.1%formic acid(A)-acetonitrile(B)in gradient elution at a flow rate of 1.0ml/min:0-5min88% A,5-10 min 88%-82% A,10-15 min 82%-70% A,15-20 min 70%-60% A.The column temperature was 25 ℃ and the detection wavelength was 280 nm.Results:The calibration curves of gentiopicroside and rosmarinic acid showed good linearity in the ranges of 6.75-431.6μg/ml(r=1.000)and 3.16-201.8μg/ml(r=1.000),respectively.The results of intra-day and interday precisions were both within 2%(n=3).The average additional recovery rates were(100.89±2.25)% and(98.19±1.13)%(n=6),respectively.Conclusion:The HPLC method proves to be simple,accurate and reproducible.It could be used for quality control of gentiopicroside and rosmarinic acid in the preparation of Fuzhengpingxiao capsules.
出处
《药学服务与研究》
CAS
2015年第6期436-438,共3页
Pharmaceutical Care and Research
关键词
龙胆苦苷
迷迭香酸
含量测定
扶正平消胶囊
色谱法
高效液相
gentiopicroside
rosmarinic acid
content determination
Fuzhengpingxiao capsule
chromatography
high performance liquid