用HPLC-MS/MS法测定人血浆中曲唑酮的浓度

Determination of trazodone in human plasma by HPLC-MS/MS method

目的：建立HPLC-MS/MS法测定人血浆中曲唑酮的浓度。方法：血浆样品经蛋白质沉淀法进行前处理后,以甲醇-水-醋酸铵-甲酸为流动相体系梯度洗脱,采用CAPCELL PAK C18MG色谱柱分离,流速为0.4ml/min,柱温为50℃。采用正离子多反应监测模式进行监测,以电喷雾离子源作为电离技术。结果：曲唑酮浓度在10.0~3000ng/ml范围内线性关系良好（r=0.996 9）,平均提取回收率为97.5%,批内、批间RSD均≤8.2%（n=6）。结论：此方法专属、灵敏、准确、简单、快速,适用于曲唑酮临床生物样本的分析和研究。

Objective：To establish a HPLC-MS/MS method for the determination of trazodone（TRZ）in human plasma.Methods：The plasma samples were treated by protein precipitation and separated in CAPCELL PAK C18 MG column with a mobile phase of methanol-water-ammonium acetate-formic acid under gradient conditions.The flow rate was 0.4 ml/min and column temperature was 50 ℃.Detection was performed in positive ion and multiple reaction monitoring（MRM）acquisition mode with electrospray ion source（ESI）as the ionization technique.Results：TRZ displayed good linearity within the ranges of10.0-3000ng/ml（r=0.996 9）.Mean extraction recovery rate was 97.5%.The intra-batch and inter-batch relative standard deviation（RSD）was≤8.2%（n=6）.Conclusion：The HPLC-MS/MS method is specific,sensitive,accurate,simple and rapid,which could be used for analysis and research of TRZ in biospecimens.