高效液相色谱-串联质谱测定牛肉粉中群勃龙

Determination of trenbolone in beef by high performance liquid chromatography tandem mass spectrometry

本试验建立了牛肉粉中孕激素群勃龙的超高效液相色谱-串联质谱测定方法,采用多反应监测方式,内标法定量。1.0g牛肉粉经4mL水复溶后,经β-葡萄糖醛酸酶/芳香基硫酸酯酶溶解,加入20mL乙腈超声提取20min,提取液经正己烷脱脂后经氨基柱净化。添加水平1μg/kg和10μg/kg时,方法回收率范围为75.2%-83.7%,相对标准偏差小于10%。牛肉粉中群勃龙的检出限为0.5μg/kg,线性范围为1μg/kg-50μg/kg。该方法可用于牛肉粉中群勃龙的快速筛查和定量分析。

A new method using super high performance liquid chromatography-tandem mass spectrometry has been established to determinetrenbolonein beef powder, the multiple reaction monitoring mode and internal standard method were used. 1.0 g beef powder was dissolved by 4 ml water,then dissolvedby the beta glucuronic acid enzyme/aromatic sulfatase. And then we added 20 ml acetonitrile ultrasonic to extract by 20 min. Hexane extracted purifieation by ami- no column after degreasing. When the The concentration of trenbolonewere added with 1 g/kg and 10 g/kg,the recovery range would to 75.2%-75.2%, and the relative standard deviation less than 10%. The detection limit oftrenbolonein Beef powder was 0.5μg/kg,linear range from 1μg/kg to 50μg/kg. This method can be used for fast beef powder in the group of drug screening and quantitative analysis.