HPLC-DAD结合交替三线性分解二阶校正法测定荔枝果肉中游离植物甾醇

Determination of Free Phytosterols in Litchi Pulp by HPLC-DAD Coupled with Second-Order Calibration Based on Alternating Trilinear Decomposition

为快速测定荔枝果肉中游离植物甾醇含量,建立高效液相色谱-二极管阵列检测器结合基于交替三线性分解算法的二阶校正方法.基本色谱条件：岛津ODS-SP柱（150 mm±4.6 mm,5 μm）,流动相体积分数95％乙腈溶液,检测波长范围为190～340 nm,柱温30℃,流速1.0 mL/min,进样量20.0 μL.结果表明, ‘兰竹’和‘乌叶’荔枝样品中菜油甾醇含量分别为24.1μg/g和27.4 μg/g,两种荔枝样品豆甾醇含量分别为25.8 μg/g和26.7 μg/g,菜油甾醇和豆甾醇加标回收率分别为（95.25±0.78）％和（96.83±1.01）％.所建立的方法简单、准确、灵敏度高且可靠,可用于检测荔枝果肉中游离植物甾醇含量.

A rapid method to quantify free phytosterols in litchi pulp was established with high performance liquid chromatography with diode array detection （HPLC-DAD） coupled with second-order calibration method based on alternating trilinear decomposition （ATLD） algorithm. The chromatographic conditions were simplified by applying second- order calibration method. The mobile phase was 95% aqueous acetonitrile （V/V） at a flow rate of 1.0 mL/min. The injection volume was 20.0 μL. The temperature of inertsil ODS-SP （150 mm × 4.6 mm, 5 μm） column was set at 30 ℃. The detection wavelengths were 190-340 nm. The average spiked recoveries of campesterol and stigmasterol in litchi pulp were （95.25 ± 0.78）% and （96.83 ± 1.01）%, respectively. The pulp of ＂Lanzhu＂ and ＂Wuye＂ litchi was detected to contain 24.1 and 27.4 btg/g campesterol, and 25.8 and 26.7 μg/g stigmasterol, respectively. The newly established method is simple, accurate, highly sensitive and reliable, and can be used to determine free phytosterols in litchi pulp.