利用高效液相色谱-蒸发光检测器法分析鮰鱼脑中磷脂组成

Determination of Phospholipids in Channel Catfish Brain by High Performance Liquid Chromatography-Evaporative Light Scattering Detector(HPLC-ELSD)

对鮰鱼脑中的磷脂进行提取纯化,利用高效液相色谱-蒸发光检测器法对鮰鱼脑的磷脂含量进行检测。结果表明:鮰鱼脑磷脂中磷脂酰胆碱含量最高,占磷脂含量的45.73%,磷脂酰乙醇胺次之,含量为17.39%,磷脂酰丝氨酸含量为5.01%,磷脂酰肌醇含量为1.20%,相对较低。磷脂标准品在各自的质量浓度检测范围内线性关系良好,并且鮰鱼脑的磷脂中各组分分离效果良好,对于其他磷脂样品的测定也具有重要的参考价值。

In this paper, the phospholipids in the brain of channel catfish were extracted and purified. A high-performance liquid chromatography equipped with an evaporative light scattering detector （HPLC-ELSD） was used to analyze the phospholipid components. The results indicated that the content of phosphatidylcholine （PC） was the highest among the detected phospholipids phospholipids in channel catfish brain, which accounted for 45.73% of the total phospholipids, followed by phosphatidylethanolamine （PE）, which accounted for 17.39%. The proportions of phosphatidylserine （PS） 1 （PI） in relative to the total phospholipids were 5.01% and 1.20%, respectively. Under optimized experimental conditions, the respective peak areas were finely fitted to a linear model within the indicated range, and each class of phospholipids was separated well. The method also can be applicable for the determination ofphospholipids in other samples.