高效液相色谱-串联质谱同时测定草莓、杨梅中20种植物生长调节剂残留

Simultaneous Determination of 20 Plant Growth Regulator Residues in Strawberry and Chinese Bayberry(Myrica rubra) by High Performance Liquid Chromatography-Tandem Mass Spectrometry

建立一种高效液相色谱-串联质谱同时测定草莓和杨梅中20种植物生长调节剂残留的分析方法.样品经含1％乙酸的乙腈溶液提取后,以Phenomenex XB-C18（100 mm×2.1mm,2.6 μm）为分析色谱柱,甲醇和5 mmol/L乙酸铵-0.1％乙酸缓冲溶液为流动相进行梯度洗脱,基质匹配标准溶液外标法定量.结果表明,20种植物调节剂在各自的质量浓度范围内线性关系良好（r≥0.990 0）,草莓和杨梅中3个添加量的回收率为70.0％～114.8％,相对标准偏差（n=5）为1.25％～11.1％,检出限在0.14～2.9 μg/kg之间.该方法操作简单、提取效果好,具有良好的灵敏度、回收率和重复性.

The present paper describes the development and validation of a method for the simultaneous determination of 20 plant growth regulator residues in strawberry and Chinese bayberry （Myrica rubra） by using high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS）. The plant growth regulator residues in 15 g of samples were extracted with acetonitrile （containing 1% acetic acid） and then analyzed using HPLC-MS/MS. The chromatographic analysis was carried out on a Phenomenex XB-C18 （100 mm× 2.1 mm, 2.6 tim） column with methanol and 5 mmol/L ammonium acetate solution containing 0.1% acetic acid as the mobile phases with a gradient program, and the analytes were quantified by matrix-matched external standard method. The recoveries of 20 plant growth regulators in strawberry and Chinese bayberry at three spiked levels were 70.0%-114.8%, with relative standard deviation （RSD） between 1.25% and 11.1%. The limits of detection of the method ranged from 0.14 to 2.9 μg/kg. The method is simple, rapid, sensitive, accurate and repeatable. These figures of merit meet the requirements for the detection of 20 plant growth regulator residues in strawberry and Chinese bayberry.