基于竞争包合作用的荧光光谱法测定盐酸普萘洛尔

Determination of Propranolol Hydrochloride by Fluorescence Spectrometry Based on Competitive Inclusion Reaction

建立了基于竞争包合作用的荧光光度法测定盐酸普萘洛尔的含量。在pH 8.4时,中性红（NR）荧光较弱,加入β-环糊精后,两者形成包合物使体系的荧光强度显著增强,而盐酸普萘洛尔能通过竞争作用将部分NR从β-环糊精的空腔中置换出来,从而使体系的荧光强度降低。通过测量体系荧光强度的降低程度可作盐酸普萘洛尔的定量测定。在激发波长为465nm和发射波长为580nm的条件下,该荧光体系的荧光强度的降低程度与盐酸普萘洛尔的质量浓度在0.10~4.0mg·L-1范围内呈线性关系,检出限（3s/k）为6μg·L-1。加标回收率在97.4%~100%之间,测定值的相对标准偏差（n=6）在0.78%~1.5%之间。本方法用于实际样品中盐酸普萘洛尔的测定,结果与传统荧光光谱法测定结果一致。

A method of fluorescence spectrometry based on competitive inclusion action was proposed for the determination of propranolol hydrochloride.In a solution of pH 8.4the weak fluorescence emitted by neutral red was found to be enhanced significantly upon the addition of appropriate amount ofβ-cyclodextrin,due to formation of an inclusion complex.Furthermore,the fluorescence intensity（measured atλex465nm andλem 580nm）of the above mentioned reaction system was found to be reduced by the addition of propranolol hydrochloride due to its substitution ofβ-CD from the inclusion complex and the extent of reduction of the fluorescence intensity was found to keep linear relationship with the values of mass concentration of propranolol hydrochloride in the range of 0.10-20mg· L-1,with value of detection limit（3s/k）of 6μg· L-1.Hence,proprandol hydrochloride can be determined by measuring the reduction of fluorescence intensity of the reaction system.Recovery and precision of the method were tested,giving values of recovery in the range of 97.4%-100%,and RSD′s（n=6）in the range of0.78%-1.5%.Substantial samples were analyzed by the proposed method,giving results in agreement with those obtained by the conventional fluorospectrometric method.