高效液相色谱法测定猪肉及血清中盐酸克伦特罗残留量

HPLC Determination of Residual Amounts of Clenbuterol in Pork and Serum

采用高效液相色谱法测定了猪肉及血清中盐酸克伦特罗残留量。样品经甲醇-盐酸混合液提取,乙二胺-N-丙基硅烷净化后,采用Zorbax Eclipse C18色谱柱分离,以甲醇-0.01mol·L^(-1)磷酸二氢钠(68+32)混合液为流动相进行洗脱,在波长270nm处进行紫外检测。盐酸克伦特罗的线性范围为0.01~5.00 mg·L^(-1),检出限(3S/N)为1.8μg·kg^(-1),加标回收率在87.4%~99.2%之间,测定值的相对标准偏差(n=5)在2.1%~2.8%之间。

HPLC was applied to the determination of residual amounts of clenbuterol in pork and serum.The sample was extracted with a mixture of methanol and 0.01 mol·L^（-1） hydrochloric acid（68＋32）and after purification with ethylenediamine-N-propylsilane,the sample solution was separated on Zorbax Eclipse C18 chromatographic column with a mixture of methanol and sodium dihydrogen phosphate solution（68＋32）as mobile phase.UV detection was made at the wavelength of 270 nm.Linearity range of clenbuterol was between 0.01-5.00mg·L^（-1）,with detection limit（3S/N）of 1.8μg·kg^（-1）.Values of recovery obtained by standard addition method were in the range of 87.4%-99.2%,with RSDs（n=5）in the range of 2.1%-2.8%.