摘要
以‘早钟6号'(黄肉)和‘白玉'(白肉)两类批把为材料,测定不同发育阶段果实果皮和果肉中类胡萝卜素含量,并对15个生物合成相关基因的表达进行了分析。随着果实发育成熟,β-胡萝卜素含量在黄肉‘早钟6号'果皮和果肉中增加,到成熟期达到最高值,分别为68.53和11.92μg·g^(-1)FW;在白肉的‘白玉'果皮中也呈增加趋势,到成熟期最高,为38.89μg·g^(-1)FW,但果肉中略有下降,从最初的0.47μg·g^(-1)FW降低至0.29μg·g^(-1)FW。两个品种果皮和果肉的β-隐黄质含量表现为持续增加,均在成熟期达到最高值。叶黄质含量在‘早钟6号'果皮中表现为下降,在果肉中则持续上升;在‘白玉'果皮中表现为先降后升,在果肉中变化不大,维持较低水平。‘早钟6号'进入转色期后,与‘白玉'相比,在果皮中的PSY和CYCB表达量较高,而在果肉中CYCB和BCH的表达量较高,提示批把不同发育阶段类胡萝卜素的积累主要受PSY、CYCB、BCH基因的共同调控。
The yellow-fleshed loquat ＇Zaozhong 6＇ and the white-fleshed loquat ＇Baiyu＇ were used as materials,the accumulation of carotenoids in peel and pulp during different fruit development stages were detected,and the expression of 15 carotenogenic genes were analysized.During loquat fruit ripening,the content of β-carotene in peel and pulp of ＇Zaozhong 6＇ increased gradually and peaked at the fully ripe stage,in the peel it was 68.53 μg·g^（-1） FW,while in the pulp it was 11.92 μg·g^（-1） FW.And in the peel of ＇Baiyu＇,the content of β-carotene increased and peaked at the fully ripe stage,reached to 38.89μg·g^（-1） FW,while it decreased in the pulp of ＇Baiyu＇,from the original 0.47 μg·g^（-1） FW reduced to 0.29μg·g^（-1） FW;The content of β-cryptoxanthin in the peel and pulp of ＇Zaozhong 6＇ and ＇Baiyu＇ both increased steadily,and peaked at the fully ripe stage;However,the content of lutein decreased in the peel of ＇Zaozhong 6＇ and increased in the pulp of ＇Zaozhong 6＇;It droped and then rised in the peel of ＇Baiyu＇,and there was no significant change of β-cryptoxanthin in the pulp of ＇Baiyu＇.After the breaker stage,the mRNA levels of phytoene synthase（PSY） and chromoplast-specific lycopene β-cyclase（CYCB） were higher in the peel,and CYCB and β-carotene hydroxylase（BCH） mRNAs were higher in the flesh of＇Zaozhong 6＇,compared with ＇Baiyu＇.The results showed that the expression level of PSY,CYCB and BCH was likely to cooperatively regulate the accumulation of carotenoids.
出处
《园艺学报》
CAS
CSCD
北大核心
2015年第11期2153-2162,共10页
Acta Horticulturae Sinica
基金
广州市科技计划项目(201504010028)
