摘要
[目的]研究三叶青快繁技术。[方法]以药用植物三叶青组培苗的节部为外植体,研究了植物生长调节剂对三叶青增殖和生根的影响。[结果]在含有6-BA 0.5 mg/L+NAA 0.1 mg/L的MS培养基中,三叶青增殖倍数最大;而三叶青的最佳生根培养基为MS+IBA 0.5 mg/L。生根的三叶青经过驯化后移栽到含有蛭石、草炭、珍珠岩(3∶6∶1)的混合基质中,一个月后成长为健康植株。[结论]该研究建立的三叶青高效快繁体系,为解决三叶青的人工栽培所需种苗提供了技术支撑。
[ Objective ] To study rapid propagation technique of Tetrastigma hemsleyanum. [ Method] Using medicinal plant Tetrastigma hemsleyanum as experimental material, the effects of plant growth regulators on Tetrastigma hemsleyanum' s multiplication and rooting were studied. [ Result] The results showed that in 6-BA 0.5 mg/L + NAA 0.1 mg/L medium; Tetrastigma hemsleyanum' s proliferation rate is the largest; Yet the best rooting medium is MS + IBA 0.5 mg/L. The plants with roots after domestication transplanted to mixed substrate containing ver- miculite, peat, perlite (3:6:1 ). After a month, it grow into a healthy plant. [ Conclusion ] This study established an efficient micropropagation system of Tetrastigma hemsleyanum, providing the technical support to solve Tetrastigma hemsleyanum' s needed for artificial cultivation of seedlings.
出处
《安徽农业科学》
CAS
2015年第35期208-209,共2页
Journal of Anhui Agricultural Sciences
基金
玉环县科技局科技攻关项目"玉环植物种苗快繁技术及其产业化"
关键词
三叶青
组织培养
增殖
生根
移栽驯化
Tetrastigma hemsleyanum
Tissue culture
Multiplication
Rooting
Domesticated cultivation