乌苏里瓦韦抑菌作用研究

Antibacterial Action of Lepisorus ussuriensis(Regel et Maack) Ching

[目的]探讨乌苏里瓦韦的抑菌作用。[方法]以乌苏里瓦韦为研究对象,在单因素的基础上,选用Na+浓度、蔗糖浓度、不同的有机溶剂萃取物,采用正交试验的原理,研究乌苏里瓦韦提取液对抑菌作用的影响。[结果]乌苏里瓦韦乙醇提取物对金黄色葡萄球菌、大肠杆菌、粪球菌、枯草杆菌、沙门氏杆菌均有抑制作用,但对枯草杆菌的抑制作用最强。最佳抑菌作用的条件为蔗糖浓度20%、Na+浓度为30%、乌苏里瓦韦乙醇提取液的石油醚萃取物,测定对枯草杆菌的抑菌环直径为8.932 mm。[结论]乌苏里瓦韦提取液具有抑菌作用。

[ Objective ] The research aimed to approach antibacterial effect of Lepisorus ussur/ens/s. [ Method ] With Lepisorus ussuriensis as the research object ,based on a single factor,using Na＋ concentration, sucrose concentration and different organic solvent extracts, the effect of the extract liquid of Lepisorus ussttriensis on bacteriostatic action was studied by the principle of ortbogonal test. [ Result] Ethanol extract liquid of Lepisorus ussuriensis had a good inhibitory effect on staphylococcus aureus, escherlchia coli ,fecal bacteria, bacillus subtilis and salmonella, especially to the bacillus subtilis. When the sucrose concentration was 20%, and Na＋ concentration was 30%, Lepisorus ussuriensis bacteriostatic had the best effect on petroleum ether extract, eventually, it turns out that the diameter of the bacteriostatic ring was 8. 932 ram. [ Conclusion]Lepisorus ussuriensis extract liquid had bactefiostatic action.