消化道逆环境对长双歧杆菌的存活及黏附力的影响

Effect of digestive tract stress on survival and adhesion ability of Bifidobacterium longum

长双歧杆菌LPL-1来源于中国长寿老人肠道,具有潜在的益生功效。为评价其应用潜力,研究探讨了长双歧杆菌LPL-1经历消化道逆环境后的存活及黏附性能。以单独及连续的消化道逆境处理双歧杆菌,以活菌数代表其存活能力,以黏附于结肠上皮癌细胞HT-29的菌数代表其黏附力。研究结果表明:胃酸溶液、胃蛋白酶、胆盐及胰蛋白酶单独处理后,初始9 log cfu/m L的双歧杆菌活菌数分别降低至7.2、6.1、7.1、7.1 log cfu/m L;口腔-食道到胃-十二指肠-回肠的连续逆境处理后,双歧杆菌的存活数量为3.1 log cfu/m L。胃酸溶液、胃蛋白酶、胆盐及胰蛋白酶单独处理后,初始9 log cfu/m L的双歧杆菌黏附于HT-29细胞的数量从67个/50个细胞降低到65.4、42.5、57.6、50.7个/50个细胞;连续消化道逆境处理后为14.1个/50个细胞。研究的结果证明了长双歧杆菌LPL-1有较强的消化道逆境耐受力,有开发为益生菌制剂的潜力。

Bifidobacterium longum LPL-1,isolated from Chinese centenarians faeces,are potential probiotic.The present study evaluated the survival and adhesion ability of B.longum LPL-1 under the stress of digestive tract.The viable account and adhesion number of B.longum LPL-1 after individual or successive stress were analyzed,as the indicator of survival and adhesion ability respectively.The results showed that：the viable count of B.longum LPL-1 decreased from 9 log cfu/m L to 7.2,6.1,7.1,7.1 log cfu/m L after gastric acid,pepsin,bile salt,trypsin treatment respectively,and to 3.1 log cfu/m L after successive digestive tract stress.On the other hand,the adhesion number of B.longum LPL-1 decreased from 67/50 cells to 65.4,42.5,57.6,50.7/50 cells after gastric acid,pepsin,bile salt,trypsin treatment respectively,and to 14.1/50 cells after successive stress.The results of the study proved that B.longum LPL-1 had strong tolerance to the stress of digestive tract,which had great application potential in probiotic products.