腺苷预处理对大鼠脊髓缺血再灌注损伤保护作用的实验研究

Protective effect of adenosine preconditioning on spinal cord ischemia-reperfusion injury in rats

目的探讨腺苷预处理对脊髓缺血再灌注损伤的保护作用。方法制作大鼠脊髓缺血再灌注损伤模型。72只SD大鼠随机分为3组:假手术组(A组)、缺血再灌注组(B组)、腺苷预处理组(C组),再按缺血再灌注后不同时间把各组随机分成3个亚组。通过BBB神经评分观察3组大鼠的神经症状;应用HE染色评价脊髓内的病理组织学变化;并测定3组大鼠不同时间点的SOD、MDA含量。结果 A组、C组在各个时间点的BBB评分明显高于B组(P<0.05);与B组相比,A组、C组细胞形态学变化轻,脊髓组织的SOD含量明显增高(P<0.05),MDA明显降低(P<0.05)。结论腺苷预处理通过提高SOD水平,降低氧自由基和脂质过氧化对大鼠脊髓缺血再灌注损伤进行保护,使神经损伤症状得到一定程度的恢复。

Objective To study the protective effect of adenosine preconditioning on spinal cord ischemia-reperfusion injury in rats. Methods The models of ischemia reperfusion injury were made. A total of 72 rats were randomly divided into three groups：sham operated group （A guoup） ,the i schemia-reperfusion group（ B group） and the adenosine preconditioning group（ C group）. The three groups were further divided into three subgroups respectively. The nerve function was evaluated by BBB scoring system at the moment of 6 h ,24 h ,48 h after the reperfusion. The tissue pathological structure was observe by HE stain through optical microscope and the content of SOD and MDA were detected. Results BBB scores of both group A and group C were significantly higher than that of group B （P 〈 0. 05 ）. Compared with group B, morphological abnormality in group A and B was alleviated and SOD in spinal tissues increased and MDA decreased （P 〈 0.05 ）. Conclusion Adenosine pre-treatment protected spinal tissues from ischemia reperfusion induced-damage by reducing oxygen free radicals and lipid over oxidation, which helps to alleviate neural damage.