摘要
目的探讨中性粒细胞明胶酶相关脂质运载蛋白(NGAL)在裸鼠原位胰腺癌组织中的表达情况以及与胰腺癌发生发展的关系。方法应用RT-PCR法检测22例裸鼠胰腺癌模型癌组织、癌旁组织和正常胰腺组织中NGAL基因及其编码蛋白的表达情况;采用免疫组化方法检测22例胰腺癌组织石蜡切片中NGAL蛋白表达情况。结果胰腺癌组织及其癌旁组织中NGAL m RNA相对表达量明显高于正常胰腺组织(P<0.05),胰腺癌组织中NGAL m RNA相对表达量又明显高于癌旁组织(P<0.05);NGAL蛋白在胰腺癌组织中的表达强阳性率以及相对表达量均明显高于癌旁组织和正常胰腺组织(P<0.05)。结论 NGAL在胰腺癌组织中呈高表达,NGAL可能是胰腺癌发生发展过程中的重要调节因子。
Objetive To study the expression of lipid associated with neutrophil gelatinase associated lipocalin （NGAL） in nude mice orthotopic pancreatic cancer tissues and the relationship between the occurred and development of pancreatic cancer. Methods The expressions of NGAL mRNA and protein of pancreatic cancer tissues and their adjacent tissues, and normal pancreatic tissues in nude mice were detected by using RT-PCR and immunohistochemical methods. Results The expressions of NGAL mRNA in pancreatic cancer tissues and adjacent tissues were significantly higher than that in normal pancreatic tissues （P〈0.05）, and the expression of NGAL mRNA in pancreatic carcinoma tissues was significantly higher than that in para carcinoma tissues （P〈0.05）. The strong positive expression rate of NGAL protein in pancreatic carcinoma tissues was significantly higher than thoes in para carcinoma tissues and normal pancreatic tissues （P〈0.05）. Conclusions NGAL is highly expressed in pancreatic cancer tissues, and NGAL may be an important regtdatory factor in the development of pancreatic cancer.
出处
《中国普外基础与临床杂志》
CAS
2016年第1期33-37,共5页
Chinese Journal of Bases and Clinics In General Surgery
基金
惠州市科学计划项目(项目编号:2012Y136)~~
