四唑染色法测定茄子种子生活力适宜条件的研究

Study on the Suitable Conditions for the Determination of Eggplant Seed Viability by TTC Method

根据四唑(2,3,5-Triphenyl Tetrazolium Chloride,缩写TTC)染色法测定种子生活力的原理,研究不同浓度四唑溶液,不同染色温度和不同染色时间对茄子种子生活力测定的影响,摸索出测定茄子种子生活力的最佳条件。结果表明,四唑溶液浓度、染色温度、染色时间三者之间存在一定的相关性,通过改变三者中的一者或两者可以达到改变其它两者或一者的目的。比较四唑染色法测定京茄黑霸种子生活力的不同条件,得出结论:处理组合23(浸种温度30℃、浸种时间4h、染色液浓度1%、染色温度35℃、染色时间18h)为最理想的处理组合,为TTC法快速测定京茄黑霸种子生活力的适宜条件。

According to the principle of tetrazolium （2,3,5-Triphenyl Tetrazolium Chloride, abbrevia- tion TTC） staining method for the determination of seed viability,research different concentration of the tetrazolium solution,staining temperature and staining time for the determination of eggplant seed viability, figuring out the optimum condition for the determination of eggplant seed viability. The results showed that there was a certain correlation among the concentration of the tetrazolium solution, staining temperature and staining time, by changing one or two of the three can achieve the purpose of changing other two or one, therefore, it＇s possible to determine the optimum condition according to the actual need.Compare different conditions of TTC method for testing Jing Qie Hei Ba eggplant seed viability.This paper put forward：processing combination 23, soaking seeds in water at 30 ℃ for 4 h,concentration of the tetrazolium solution is 1%,staining at 35 ℃ for 18 h,is the optimum processing combination,which is the suitable TTC method for rapid determination of Jing Qie Hei Ba eggplant seed viability.