响应曲面法优化金银花多糖提取条件及抗氧化活性研究

A Study of Optimization of Extraction Conditions and Antioxidant Activity for Polysaccharide from Honeysuckle by Response Surface Methodology

文章采用响应曲面法优化超声提取金银花多糖的条件,研究金银花多糖的抗氧化活性。依据响应曲面法的中心组合设计,以提取时间、p H及液料比为自变量,研究各自变量及其交互作用对多糖提取率的影响。考察金银花多糖对·DPPH自由基和·OH自由基的清除效果。金银花多糖的最佳提取条件:时间40min、液料比30:1(m L/g),p H值为4,此时金银花多糖最大提取率达1.92%。金银花多糖对·DPPH自由基和·OH自由均有较强的清除能力,其EC50分别为0.016mg/m L和0.21mg/m L。该优化条件稳定、可行,实验结果准确可靠;金银花多糖对·OH和·DPPH自由基均有较理想的清除能力。

Response Surface Methodology（RSM）optimizes the best ultrasonic extraction condition of polysaccharides from honeysuckle and studies its antioxidant activity. Based on the central composite design of RSM,the extraction time,p H and the solid-liquid ratio are extracted,and the effects of the independent variables and their interaction on the extraction yield of polysaccharide are investigated. Respectively studied the scavenge ability of honeysuckle polysaccharide on ·DPPH free radicals and ·OH free radical. With the best extraction conditions of 40 min,30：1（m L/g） liquid-solid ratio and 4 PH value,the honeysuckle polysaccharide extraction yield was up to 1.92%. Honeysuckle polysaccharide had strong scavenge capacity to·DPPH and·OH,the EC50 are 0.016 mg/ml and 0.21 mg/ml respectively. The optimized process is stable and feasible,and the experimental results are accurate and reliable.